Name: SARS-COV-2 Spike S1 (N501Y) Protein (COV-VM1SY)
Brand: KactusBio
SKU: COV-VM1SY-100UG
Price: 330.0 USD
Availability: InStock

SARS-COV-2 Spike S1 (N501Y) Protein (COV-VM1SY)

Immobilized SARS-COV-2 Spike S1 (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 66.2ng/ml determined by ELISA.

SARS-COV-2 Spike S1 (N501Y) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of SARS-COV-2 Spike S1 (N501Y) is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized SARS-COV-2 Spike S1 (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 66.2ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Val16-Arg685

Catalog #COV-VM1SY

Size

100UG

500UG

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Product Description	Recombinant SARS-COV-2 Spike S1 (N501Y) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val16-Arg685.
Product Background	A new variant of SARS-CoV-2 is spreading in the UK and is rapidly becoming a global threat.It is characterised by multiple mutations in the spike protein. Among them, N501Y is of major concern because it involves one of the six key amino acid residues determining a tight interaction of the SARS-CoV-2 receptor-binding domain (RBD) with its cellular receptor angiotensin-converting enzyme 2 (ACE2).
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	Spike S1
Synonyms	S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
Accession	YP_009724390.1
Species	SARS-COV-2
Biotinylated	no
Amino Acid Range	Val16-Arg685
Molecular Weight	The protein has a predicted MW of 76 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result.
Activity	Immobilized SARS-COV-2 Spike S1 (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 66.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized SARS-COV-2 Spike S1 (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 66.2ng/ml determined by ELISA.
	SARS-COV-2 Spike S1 (N501Y) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of SARS-COV-2 Spike S1 (N501Y) is greater than 90% as determined by SEC-HPLC.