SARS-COV-2 Spike S1 (N501Y) Protein (COV-VM1SY)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS-COV-2 Spike S1 (N501Y) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2 , hFc Tag with the EC50 of 80.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Val16-Arg685

Catalog: COV-VM1SY

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Product Description Recombinant SARS-COV-2 Spike S1 (N501Y) Protein is expressed from Expi293 with His tag at the C-terminal.It contains Val16-Arg685.
Product Category Recombinant Protein
Target Spike S1 (N501Y)
Synonyms S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
Gene/Protein ID YP_009724390.1
Species SARS-COV-2
Exact Sequence Val16-Arg685
Molecular Weight The protein has a predicted MW of 76 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.
Activity Immobilized SARS-COV-2 Spike S1 (N501Y) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2 , hFc Tag with the EC50 of 80.3ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized SARS-COV-2 Spike S1 (N501Y) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2 , hFc Tag with the EC50 of 80.3ng/ml determined by ELISA.
The purity of SARS-COV-2 Spike S1 (N501Y) is greater than 95% as determined by SEC-HPLC.
SARS-COV-2 Spike S1 (N501Y) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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