Name: SARS-COV-2 Spike S1 (N501Y) Protein (COV-VM1SY)
Brand: KactusBio
SKU: COV-VM1SY-100UG
Price: 300.0 USD
Availability: InStock

SARS-COV-2 Spike S1 (N501Y) Protein (COV-VM1SY)

Immobilized SARS-COV-2 Spike S1 (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 66.2ng/ml determined by ELISA.

SARS-COV-2 Spike S1 (N501Y) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of SARS-COV-2 Spike S1 (N501Y) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized SARS-COV-2 Spike S1 (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 66.2ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Val16-Arg685

Catalog #COV-VM1SY

Size

100UG

500UG

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Product Description	Recombinant SARS-COV-2 Spike S1 (N501Y) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val16-Arg685.
Product Background	A new variant of SARS-CoV-2 is spreading in the UK and is rapidly becoming a global threat.It is characterised by multiple mutations in the spike protein. Among them, N501Y is of major concern because it involves one of the six key amino acid residues determining a tight interaction of the SARS-CoV-2 receptor-binding domain (RBD) with its cellular receptor angiotensin-converting enzyme 2 (ACE2).
Product Category	Recombinant Protein / Antibody discovery

Protein	Spike S1 (N501Y)
Synonyms	S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
Accession	YP_009724390.1
Species	SARS-COV-2
Biotinylated	no
Amino Acid Range	Val16-Arg685
Molecular Weight	The protein has a predicted MW of 76 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result.
Activity	Immobilized SARS-COV-2 Spike S1 (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 66.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized SARS-COV-2 Spike S1 (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 66.2ng/ml determined by ELISA.
	SARS-COV-2 Spike S1 (N501Y) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of SARS-COV-2 Spike S1 (N501Y) is greater than 95% as determined by SEC-HPLC.