SARS-COV-2 Spike S1 (N501Y) Protein (COV-VM1SY)
Catalog: COV-VM1SY
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Product Description | Recombinant SARS-COV-2 Spike S1 (N501Y) Protein is expressed from Expi293 with His tag at the C-terminal.It contains Val16-Arg685. |
Product Category | Recombinant Protein |
Target | Spike S1 (N501Y) |
Synonyms | S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1 |
Gene/Protein ID | YP_009724390.1 |
Species | SARS-COV-2 |
Exact Sequence | Val16-Arg685 |
Molecular Weight | The protein has a predicted MW of 76 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized SARS-COV-2 Spike S1 (N501Y) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2 , hFc Tag with the EC50 of 80.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag | C-His |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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