Background
Major Histocompatibility Complex (MHC) is a highly polymorphic family of cell surface proteins, also known as HLA in humans. MHC binds to peptides derived from degraded intracellular antigens to form MHC-peptide complexes, which are then transported to the cell surface. These MHC complexes are recognized by corresponding T cell receptors (TCR) to initiate an immune response. In humans, the MHC complexes involved in antigen presentation are mainly MHC class I and MHC class II. MHC class I, when bound to antigenic peptides, is recognized by CD8+ T cells, while MHC class II, when bound to antigenic peptides, is recognized by CD4+ T cells. Targeting MHC complexes can achieve the killing of tumor cells.
KACTUS has successfully developed a series of MHC complexes, including monomers, tetramers, chimeric MHCs, and peptide-ready MHCs, as well as related custom services, fully supporting the development of tumor immunotherapy drugs. Our MHC complex portfolio covers popular targets such as NY-ESO-1, KRAS, AFP, and HPV16 which can be used to meet the diverse needs of immunology, molecular screening, TCR affinity validation, blocking assays, and other applications.
Product Features
Catalog Products
Our extensive catalog includes a wide range of MHC monomers and tetramers that are readily available to meet your research needs. Whether you are studying antigen presentation or T cell responses, our products are designed to provide reliable and consistent results.
Choice of Allele
We offer a diverse selection of alleles to ensure you can find the specific MHC molecules required for your studies. Our alleles cover various species and haplotypes, enabling precise and comprehensive immunological research.
Verified Bioactivity
Each of our MHC monomers and tetramers undergoes rigorous testing to verify bioactivity. This ensures that our products will perform as expected in your assays, providing you with confidence in your results.
Biotinylation & Fluorescent Labels
Enhance your experiments with our biotinylated and fluorescently labeled MHC monomers and tetramers. These options provide flexibility in detection methods, allowing you to choose the best approach for your flow cytometry, microscopy, or other analytical techniques.
Browse by Allele
MHC complexes play a crucial role in the immune system by presenting antigenic peptides to cytotoxic CD8+ T cells (class I) or helper CD4+ T Cells (class II).
MHC I are heterotrimers consisting of a transmembrane MHC heavy chain, a light chain known as β2-microglobulin (β2m), and an 8-10 peptide antigen. The heavy chain contains two peptide binding domains (α1 and α2), an immunoglobulin-like domain (α3), and a transmembrane region. The folding of the α1 and α2 domains forms a groove where peptide antigens bind to the MHC I complex. β2m stabilizes the peptide binding groove and MHC I presentation.
MHC II are heterodimers composed of two transmembrane proteins: an α chain and a β chain. Each chain has two extracellular domains (α1 and α2 for the α chain, and β1 and β2 for the β chain), a transmembrane segment, and a cytoplasmic tail. The α1 and β1 domains form a peptide-binding groove where peptides, typically 13-18 amino acids long, bind. This binding groove is more open-ended compared to MHC I, allowing for longer peptides.
Class I Alleles - Human
Other Alleles
For other class I or class II alleles, or non-classical alleles, try our custom MHC services.
Browse by Antigenic Peptide
MHC binds peptide fragments of intracellular antigens to form MHC-peptide complexes, which are transported to the cell surface and recognized by T cell receptors (TCRs) to initiate an immune response. Each nucleated cell expresses 5x10⁵ copies of each MHC I, presenting various peptides simultaneously to cytotoxic T cells. Their unique binding with TCRs is crucial for adaptive immunity and TCR-related therapies like TCR-T development. Genomic mutations in cancers produce tumor-specific peptide antigens (neoantigens), presented by MHC, which enable the immune system to target cancer cells.
Custom Peptide
For MHCs with custom peptide sequences, submit our Custom MHC Request Form.
Alternatively, try a Peptide-Ready MHC to load your peptide in-house onto an MHC tetramer or monomer.
Product Validation Data
We've demonstrated via ELISA and SPR binding assays that our MHC-peptide complexes and loaded peptide-ready MHCs have good binding affinity to TCRs. Our MHC products are well-suited for applications such as antibody binding, affinity research, TCR screening, and blocking assays.
HLA-A*02:01 Tetramer & FMNKIYEI
Figure 1. Immobilized HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR at 2µg/ml (100ul/well) on the plate can bind HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer, His Tag with an EC50 of 26.6ng/ml determined by ELISA.
Biotinylated HLA-A*02:01 Monomer & SLLMWITQV
Figure 2. Immobilized Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Monomer, His Tag with the EC50 of 31.3ng/ml determined by ELISA.
HLA-G Tetramer & RIIPRHLQL
Figure 3. Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer with an affinity constant of 4.62 nM as determined in SPR assay (Biacore T200).
HLA-A*02:01 Tetramer & YLEPGPVTA
Figure 4. In SPR analysis, the GP100 TCR & Anti-CD3 Bispecific Fusion can bind to the HLA-A*02:01&B2M&GP100 (YLEPGPVTA) complex tetramer, with an affinity constant of 0.196 nM.
HLA-A*02:01 Monomer & FMNKFIYEI
Figure 5. Human HLA-A*02:01&B2M&AFP (FMNKFIEl) Monomer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR with an affinity constant of 0.3991 µM as determined in SPR assay (Biacore T200).
HLA-A*02:01 Tetramer & FMNKFIYEI
Figure 6. Human HLA-A*02:01&B2M&AFP (FMNKFIEl) Tetramer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR with an affinity constant of 0.111 µM as determined in SPR assay (Biacore T200).
PE-SA Assembled Tetramer with SIINFEKL, OT1 Mouse Whole Blood
Figure 7. Flow Cytometry Analysis of OT1 Mouse Whole Blood Cells and T Cells Using PE-SA Assembled Tetramers. The right panel displays the binding of OT1 mouse T cells to the Mouse H-2K(b) & B2M & OVA (SIINFEKL) PE-SA assembled tetramer, with a significant shift in fluorescence and higher percentage of MHC PE-H positive cells (39.60% in Q5-4), compared to the left control, demonstrating effective binding and tetramer assembly.
PE-SA Assembled Tetramer with SIINFEKL, Peptide-Pulsed OT1 Mouse Spleen Blood
Figure 8. Flow Cytometry Analysis of SIINFEKL Peptide-Pulsed OT1 Mouse Spleen Cells Using PE-SA Assembled Tetramers. The right panel displays the binding of OT1 mouse spleen cells to the custom-assembled tetramer (Mouse H-2K(b) & B2M & OVA (SIINFEKL) PE-SA), with a significant shift in fluorescence and higher percentage of MHC PE-H positive cells (97.41% in Q5-4), demonstrating effective binding and tetramer assembly.
Available Products
Browse our full catalog of 200+ major histocompatibility complex products, including monomers, tetramers, biotinylation, and fluorescent labeling. Our MHC products are mammalian-expressed and bioactivity-verified. Order an off-the-shelf MHC product or request a custom MHC complex.
Customize your MHC
Our team can modify parameters on an existing MHC class I or class II catalog product such as neoantigen peptide, biotinylation, and fluorescent-labeling. Alternatively, submit our custom MHC request form to order an entirely customized MHC complex. We'll work closely with you to make sure the MHC complex meets your unique specifications.
Custom MHC Services
Soluble TCR Expression
Custom expression of soluble TCR with TCR/MHC binding analysis via SPR.
Peptide-ready MHCs
Load your own peptide in-house onto a stabilized monomer or tetramer
HLA-G & LILRs
Learn more about our full portfolio of HLA-G and LILRA/LILRB proteins.
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Major Histocompatibility Complex | KACTUS