Human AFP (HLA-A*02:01) Tetramer Protein (MHC-HM407T)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human AFP (HLA-A*02:01) Tetramer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-AFP (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and FMNKFIYEI peptide

Catalog: MHC-HM407T

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Product Description Recombinant Human AFP (HLA-A*02:01) Tetramer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and FMNKFIYEI peptide.
Product Category Recombinant Protein
Target AFP (HLA-A*02:01) Tetramer
Synonyms Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-feto; AFP; HPAFP; AFPD; FETA; MHC
Gene/Protein ID A0A140T913(HLA-A*02:01)&P61769(B2M)&FMNKFIYEI
Species Human
Exact Sequence Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and FMNKFIYEI peptide
Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Activity Immobilized Human AFP (HLA-A*02:01) Tetramer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-AFP (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 9ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human AFP (HLA-A*02:01) Tetramer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-AFP (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 9ng/ml determined by ELISA.
The purity of Human AFP (HLA-A*02:01) Tetramer is greater than 95% as determined by SEC-HPLC.
Human AFP (HLA-A*02:01) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

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