Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein (MHC-HM407T)
Catalog: MHC-HM407T
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| Product Description | Recombinant Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and FMNKFIYEI peptide. |
Product Background |
Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response. |
Product Category |
Recombinant Protein |
Target |
HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer |
| Synonyms | Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-feto; AFP; HPAFP; AFPD; FETA; MHC |
| MHC Form | Tetramer |
| Allele | HLA-A*02:01 |
Gene/Protein ID |
A0A140T913(HLA-A*02:01)&P61769(B2M)&FMNKFIYEI |
Species |
Human |
Exact Sequence |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FMNKFIYEI peptide |
| Molecular Weight | The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
Activity |
Immobilized Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&AFP (FMNKFIYEI) Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&AFP (FMNKFIYEI) Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA (QC Test). |
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Immobilized HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR at 2ug/ml (100ul/well) on the plate. Dose response curve for Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer, His Tag with the EC50 of 26.6ng/ml determined by ELISA. |
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The purity of Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer is greater than 95% as determined by SEC-HPLC. |
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Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%. |
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