Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein (HLG-HM41CT)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-G Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA (QC Test). The affinity constant of 4.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-G),Ile21-Met119(B2M)and RIIPRHLQL peptide

Catalog: HLG-HM41CT

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Product Description Recombinant Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide.
Product Category Recombinant Protein
Target HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer
Synonyms HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G
Gene/Protein ID P17693-1(HLA-G)&P61769(B2M)&RIIPRHLQL
Species Human
Exact Sequence Gly25-Thr305(HLA-G),Ile21-Met119(B2M)and RIIPRHLQL peptide
Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Activity Immobilized Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-G Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA (QC Test). The affinity constant of 4.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer with an affinity constant of 4.62 nM as determined in SPR assay (Biacore T200).
Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag immobilized on CM5 Chip can bind Human LILRB2 Domain1&2, His Tag with an affinity constant of 6.5uM as determined in a SPR assay (Biacore T200).
Immobilized Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-G Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA (QC Test).
Serial dilutions of Anti-LILRB2 Antibody were added into Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag : Human LILRB2, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.11ug/ml.
The purity of Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer is greater than 95% as determined by SEC-HPLC.
Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

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