Human HLA-G Complex Tetramer Protein (HLG-HM41CT)
Catalog: HLG-HM41CT
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| Product Description | Recombinant Human HLA-G Complex Tetramer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin.; It contains Gly25-Thr305 (HLA-G), Ile21-Met119 (B2M) and RIIPRHLQL peptide. |
| Product Category | Recombinant Protein |
| Target | HLA-G Complex Tetramer |
| Synonyms | HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G |
| Gene/Protein ID | P17693-1 (HLA-G)&P61769 (B2M)&RIIPRHLQL |
| Species | Human |
| Exact Sequence | Gly25-Thr305 (HLA-G), Ile21-Met119 (B2M) and RIIPRHLQL peptide |
| Molecular Weight | The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 4.62 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-G Complex Tetramer with an affinity constant of 4.62 nM as determined in SPR assay (Biacore T200). |
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Human HLA-G Complex Tetramer, His Tag immobilized on CM5 Chip can bind Human LILRB2 Domain1&2, His Tag with an affinity constant of 6.5μM as determined in a SPR assay (Biacore T200). |
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Serial dilutions of Anti-LILRB2 Antibody were added into HLA-G Complex Tetramer, His Tag : Human LILRB2, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 70.0ng/ml. |
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The purity of Human HLA-G Complex Tetramer is greater than 95% as determined by SEC-HPLC. |
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Human HLA-G Complex Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%. |
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