Human P53 R175H (HLA-A*02:01) Protein (MHC-HM415)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-P53 R175H (HLA-A*02:01) Antibody, hFc Tag at 1_g/ml (100_l/well) on the plate. Dose response curve for Human P53 R175H (HLA-A*02:01) , His Tag with the EC50 of 0.19_g/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and HMTEVVRHC peptide

Catalog: MHC-HM415

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Product Description Recombinant Human P53 R175H (HLA-A*02:01) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and HMTEVVRHC peptide.
Product Category Recombinant Protein
Target P53 R175H (HLA-A*02:01)
Synonyms MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53
Gene/Protein ID A0A140T913(HLA-A*02:01)&P61769(B2M)& HMTEVVRHC
Species Human
Exact Sequence Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and HMTEVVRHC peptide
Molecular Weight The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-P53 R175H (HLA-A*02:01) Antibody, hFc Tag at 1_g/ml (100_l/well) on the plate. Dose response curve for Human P53 R175H (HLA-A*02:01) , His Tag with the EC50 of 0.19_g/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-P53 R175H (HLA-A*02:01) Antibody, hFc Tag at 1_g/ml (100_l/well) on the plate. Dose response curve for Human P53 R175H (HLA-A*02:01) , His Tag with the EC50 of 0.19_g/ml determined by ELISA.
The purity of Human P53 R175H (HLA-A*02:01) is greater than 95% as determined by SEC-HPLC.
Human P53 R175H (HLA-A*02:01) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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