Name: Human Peptide Ready HLA-A*02:01&B2M Monomer-Protein (MHC-HM43R)
Brand: KactusBio
SKU: MHC-HM43R-100UG
Price: 341.0 USD
Availability: InStock

HPLC chromatogram displaying a sharp, dominant peak at retention time 9.107 minutes, indicating high purity of the Human Peptide Ready HLA-A*02:01&B2M Monomer-Protein sample.

Human Peptide Ready HLA-A*02:01&B2M Monomer-Protein (MHC-HM43R)

Immobilized Human Peptide Ready HLA-A*02:01&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc tag with the EC50 of 2.5ng/ml determined by ELISA.

Human Peptide Ready HLA-A*02:01&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human Peptide Ready HLA-A*02:01&B2M Monomer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Peptide Ready HLA-A*02:01&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc tag with the EC50 of 2.5ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*02:01) & Ile21-Met119(B2M)

Catalog #MHC-HM43R

Size

100UG

500UG

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Human Peptide Ready HLA-A*02:01&B2M Monomer-Protein (MHC-HM43R) Summary

Product Description	Recombinant Human Peptide Ready HLA-A02:01&B2M Monomer-Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A02:01) and Ile21-Met119(B2M).
Product Background	Peptide Ready HLA-A02:01&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A02:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
Product Category	Recombinant Protein / Antibody discovery / MHC / Peptide-ready MHCs

Peptide Ready HLA-A*02:01&B2M Specifications

Protein	Peptide Ready HLA-A*02:01&B2M
Synonyms	MHC; HLA-A*02:01; Peptide Ready
MHC Form	Monomer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)
Species	Human
Biotinylated	no
Amino Acid Range	Gly25-Thr305(HLA-A*02:01) & Ile21-Met119(B2M)
Molecular Weight	The protein has a predicted MW of 48.10 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Peptide Ready HLA-A*02:01&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc tag with the EC50 of 2.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human Peptide Ready HLA-A*02:01&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc tag with the EC50 of 2.5ng/ml determined by ELISA.
	Human Peptide Ready HLA-A*02:01&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Peptide Ready HLA-A*02:01&B2M Monomer is greater than 95% as determined by SEC-HPLC.