Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer-Protein (MHC-HM44RB)
Catalog: MHC-HM44RB
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| Product Description | Recombinant Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer-Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*03:01) and Ile21-Met119(B2M). |
Product Background |
Peptide Ready HLA-A*03:01&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*03:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner. |
Product Category |
Recombinant Protein |
Target |
Peptide Ready HLA-A*03:01&B2M Monomer |
| MHC Form | Monomer |
| Allele | HLA-A*03:01 |
Gene/Protein ID |
NP_002107.3(HLA-A*03:01)&P61769(B2M) |
Species |
Human |
Exact Sequence |
Gly25-Thr305(HLA-A*03:01) & Ile21-Met119(B2M) |
| Molecular Weight | The protein has a predicted MW of 48.40 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Liquid |
| Shipping | Shipped with dry ice. |
| Formulation | Supplied as 0.22um filtered solution in PBS (pH 7.4). |
| Stability And Storage | Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. |
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The purity of Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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