Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer-Protein (MHC-HM44RB)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-A*03:01) & Ile21-Met119(B2M)

Catalog: MHC-HM44RB

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Product Description Recombinant Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer-Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*03:01) and Ile21-Met119(B2M).

Product Background

Peptide Ready HLA-A*03:01&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*03:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Product Category

Recombinant Protein

Target

Peptide Ready HLA-A*03:01&B2M Monomer
MHC Form Monomer
Allele HLA-A*03:01

Gene/Protein ID

NP_002107.3(HLA-A*03:01)&P61769(B2M)

Species

Human

Exact Sequence

Gly25-Thr305(HLA-A*03:01) & Ile21-Met119(B2M)
Molecular Weight The protein has a predicted MW of 48.40 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA.
The purity of Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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