Biotinylated Human CTLA-4/CD152 Protein (CTL-HM401B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human CTLA-4, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human B7-1, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Lys36-Asp161

Catalog: CTL-HM401B

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Product Description Recombinant Biotinylated Human CTLA-4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys36-Asp161.

Product Background

 CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.

Product Category

 Recombinant Protein

Target

 CTLA-4/CD152
Synonyms CTLA4; CD152; ICOS; CELIAC3; GRD4; GSE; ALPS5; IDDM12

Gene/Protein ID

 P16410

Species

 Human

Exact Sequence

 Lys36-Asp161
Molecular Weight The protein has a predicted MW of 16.3 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Biotinylated Human CTLA-4, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human B7-1, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human CTLA-4, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human B7-1, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test).
Immobilized Anti-CTLA-4 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CTLA-4, His Tag with the EC50 of 45.7ng/ml determined by ELISA.
The purity of Biotinylated Human CTLA-4 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CTLA-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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