Product Description |
Recombinant Biotinylated Human CTLA-4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys36-Asp161. |
Product Background |
CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells. |
Product Category |
Recombinant Protein |
Target |
CTLA-4/CD152 |
Synonyms |
CTLA4; CD152; ICOS; CELIAC3; GRD4; GSE; ALPS5; IDDM12 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Lys36-Asp161 |
Molecular Weight |
The protein has a predicted MW of 16.3 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human CTLA-4, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human B7-1, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human CTLA-4, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human B7-1, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test). |
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Immobilized Anti-CTLA-4 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CTLA-4, His Tag with the EC50 of 45.7ng/ml determined by ELISA. |
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Biotinylated Human CTLA-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human CTLA-4 is greater than 95% as determined by SEC-HPLC. |
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