Biotinylated Human CTLA-4/CD152 Protein (CTL-HM401B)
Catalog: CTL-HM401B
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| Product Description | Recombinant Biotinylated Human CTLA-4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys36-Asp161. |
Product Background |
CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells. |
Product Category |
Recombinant Protein |
Target |
CTLA-4/CD152 |
| Synonyms | CTLA4; CD152; ICOS; CELIAC3; GRD4; GSE; ALPS5; IDDM12 |
Gene/Protein ID |
P16410 |
Species |
Human |
Exact Sequence |
Lys36-Asp161 |
| Molecular Weight | The protein has a predicted MW of 16.3 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human B7-1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CTLA-4, His Tag with the EC50 of 0.28ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human B7-1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CTLA-4, His Tag with the EC50 of 0.28ug/ml determined by ELISA. |
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Immobilized Anti-CTLA-4 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CTLA-4, His Tag with the EC50 of 45.7ng/ml determined by ELISA (QC Test). |
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The purity of Biotinylated Human CTLA-4 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human CTLA-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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