Cynomolgus/Rhesus macaque CD19 Protein (CD1-CM119)
Cynomolgus/Rhesus macaque CD19 Protein (CD1-CM119)
Cynomolgus/Rhesus macaque CD19 Protein (CD1-CM119)
Cynomolgus/Rhesus macaque CD19 Protein (CD1-CM119)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: >95% as determined by Bis-Tris PAGE
  • Activity: The affinity constant of 0.46 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln21-Trp291
Catalog #CD1-CM119
Regular price $315.00
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Product Description

Recombinant Cynomolgus/Rhesus macaque CD19 Protein is expressed from HEK293 with His tag at the N-terminus; It contains Gln21-Trp291.

Product Background

CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2+ stores.

Product Category

Recombinant Protein

Protein

CD19

Synonyms

CD19; B4; CVID3; Leu-12; MGC12802

Accession

Species

Cynomolgus/Rhesus macaque

Biotinylated

no

Amino Acid Range

Gln21-Trp291

Molecular Weight

The protein has a predicted MW of 30.96 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

Activity

The affinity constant of 0.46 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-His

Expression System

HEK293

Purity

>95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus/Rhesus macaque CD19, His Tag with an affinity constant of 0.46 uM as determined in SPR assay (Biacore T200).
Cynomolgus/Rhesus macaque CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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