Name: Human CD19 Protein (CD1-HM119)
Brand: KactusBio
SKU: CD1-HM119-100UG
Price: 412.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human CD19, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 5.3 ng/ml determined by ELISA (QC Test). The affinity constant of 0.50 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Pro20-Lys291

Catalog #CD1-HM119

Size

100UG

500UG

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Product Description	Recombinant Human CD19 Protein is expressed from HEK293 with His tag at the C-Terminus; It contains Pro20-Lys291.
Product Background	CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	CD19
Synonyms	CD19; B4; CVID3; Leu-12; MGC12802
Accession	P15391-1
Species	Human
Biotinylated	no
Amino Acid Range	Pro20-Lys291
Molecular Weight	The protein has a predicted MW of 34.7 kDa. Due to glycosylation, the protein migrates to 50-60 KDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD19, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 5.3 ng/ml determined by ELISA (QC Test). The affinity constant of 0.50 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human CD19, His Tag with an affinity constant of 0.50 nM as determined in SPR assay (Biacore T200).
	Immobilized Human CD19, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 5.3 ng/ml determined by ELISA (QC Test).
	Immobilized Human CD19, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for FMC63, mFc Tag with the EC50 of 10.0 ng/ml determined by ELISA (QC Test).
	Immobilized Human CD19, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Inebilizumab, hFc Tag with the EC50 of 11.5 ng/ml determined by ELISA.
	Immobilized Human CD19, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Denintuzumab, hFc Tag with the EC50 of 2.1 ng/ml determined by ELISA.
	Human CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Denintuzumab, hFc Tag on ProA-Biosensor, can bind Human CD19, His Tag with an affinity constant of 3.73 nM as determined in BLI assay (Gator).

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