Human CD19 Protein (CD1-HM119)
Catalog: CD1-HM119
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| Product Description | Recombinant Human CD19 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Pro20-Lys291. |
| Product Category | Recombinant Protein |
| Target | CD19 |
| Synonyms | CD19; B4; CVID3; Leu-12; MGC12802 |
| Gene/Protein ID | P15391-1 |
| Species | Human |
| Exact Sequence | Pro20-Lys291 |
| Molecular Weight | The protein has a predicted MW of 34.7 kDa. Due to glycosylation, the protein migrates to 50-60 KDa based on Bis-Tris PAGE result. |
| Activity | Immobilized Human CD19, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human CD19, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA. |
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Immobilized Human CD19, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Inebilizumab, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA. |
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Human CD19 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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