- Express System: HEK293
- Product Tag: C-His
- Purity: >95% as determined by Bis-Tris PAGE
- Activity: Immobilized Human CD19, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 5.3 ng/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: Pro20-Lys291
Product Description |
Recombinant Human CD19 Protein is expressed from HEK293 with His tag at the C-Terminus; It contains Pro20-Lys291. |
Product Background |
CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores. |
Product Category |
Recombinant Protein |
Target |
CD19 |
Synonyms |
CD19; B4; CVID3; Leu-12; MGC12802 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Pro20-Lys291 |
Molecular Weight |
The protein has a predicted MW of 34.7 kDa. Due to glycosylation, the protein migrates to 50-60 KDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD19, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 5.3 ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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