Human CD19 Protein (CD1-HM119)
Human CD19 Protein (CD1-HM119)
Human CD19 Protein (CD1-HM119)
Human CD19 Protein (CD1-HM119)
Human CD19 Protein (CD1-HM119)
Human CD19 Protein (CD1-HM119)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: >95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CD19, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 5.3 ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Pro20-Lys291
Catalog #CD1-HM119
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Product Description
 Recombinant Human CD19 Protein is expressed from HEK293 with His tag at the C-Terminus; It contains Pro20-Lys291.
Product Background
 CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores.
Product Category
 Recombinant Protein
Target
 CD19
Synonyms
 CD19; B4; CVID3; Leu-12; MGC12802
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Pro20-Lys291
Molecular Weight
 The protein has a predicted MW of 34.7 kDa. Due to glycosylation, the protein migrates to 50-60 KDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD19, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 5.3 ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD19, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 5.3 ng/ml determined by ELISA (QC Test).
Immobilized Human CD19, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Inebilizumab, hFc Tag with the EC50 of 11.5 ng/ml determined by ELISA.
Immobilized Human CD19, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for FMC63, mFc Tag with the EC50 of 10.0 ng/ml determined by ELISA (QC Test).
Immobilized Human CD19, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Denintuzumab, hFc Tag with the EC50 of 2.1 ng/ml determined by ELISA.
Human CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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