Product Description |
Recombinant Human DLL3 is expressed from HEK293 with His tag at the N-Terminus; It contains Ala27-Arg490. |
Product Background |
Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis and may be required to divert neurons along a specific differentiation pathway. It plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. |
Product Category |
Recombinant Protein |
Target |
DLL3 |
Synonyms |
Delta3; DLL3; Pudgy; SCDO1; SCDO1delta3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala27-Arg490 |
Molecular Weight |
The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human DLL3, His Tag at 1 ug/mL (100 ul/well). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 1.2 ng/ml determined by ELISA (QC Test). The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 200 mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Anti-DLL3 Antibody captured on CM5 Chip via Protein A can bind Human DLL3, His Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Human DLL3, His Tag at 1 ug/mL (100 ul/well). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 1.2 ng/ml determined by ELISA (QC Test). |
|
|
Human DLL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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