Human DLL3 Protein (DLL-HM103)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human DLL3, His Tag at 0.1 ug/mL (100 ul/well). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 4.5 ng/ml determined by ELISA (QC Test). The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ala27-Arg490

Catalog: DLL-HM103

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Product Description	Recombinant Human DLL3 is expressed from HEK293 with His tag at the N-Terminus; It contains Ala27-Arg490.
Product Background	Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis and may be required to divert neurons along a specific differentiation pathway. It plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm.
Product Category	Recombinant Protein

Target	DLL3
Synonyms	Delta3; DLL3; Pudgy; SCDO1; SCDO1delta3
Accession	Q9NYJ7-1
Species	Human
Amino Acid Range	Ala27-Arg490
Molecular Weight	The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human DLL3, His Tag at 0.1 ug/mL (100 ul/well). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 4.5 ng/ml determined by ELISA (QC Test). The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 200 mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Anti-DLL3 Antibody captured on CM5 Chip via Protein A can bind Human DLL3, His Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
	Immobilized Human DLL3, His Tag at 0.1 ug/mL (100 ul/well). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 4.5 ng/ml determined by ELISA (QC Test).
	Human DLL3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.