Name: Biotinylated Human Her2/ErbB2 Protein (HER-HM402B)
Brand: KactusBio
SKU: HER-HM402B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human Her2/ErbB2 Protein (HER-HM402B)

Immobilized Biotinylated Human Her2, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-HER2 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA (QC Test).

Biotinylated Human Her2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Her2 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human Her2, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-HER2 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Thr23-Thr652

Catalog #HER-HM402B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human Her2/ErbB2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr23-Thr652.
Product Background	ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	Her2/ErbB2
Synonyms	CD340; EGFR2; ErbB2; HER2; HER-2; herstatin; MLN 19; MLN19; NGL; TKR1; ERBB2; NEU; ENV; ENVW; ERVWE1; HERV7Q; HERVW
Accession	P04626-1
Species	Human
Biotinylated	yes
Amino Acid Range	Thr23-Thr652
Molecular Weight	The protein has a predicted MW of 72.3 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Her2, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-HER2 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human Her2, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-HER2 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA (QC Test).
	Biotinylated Human Her2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Her2 is greater than 95% as determined by SEC-HPLC.