• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human CD19, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Coltuximab, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.64 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Glu21-Lys291
Catalog #CD1-HMR102
Price
Regular price $660.00
Regular price Sale price $660.00
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human CD19 Protein (CD1-HMR102) Summary

Product Description

 Recombinant Biotinylated Human CD19 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Glu21-Lys291.

Product Background

 CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores.

Product Category

 Recombinant Protein / Antibody discovery / CAR-T targets, ADC targets

Biotinylated Human CD19 Protein (CD1-HMR102) Specifications/Details

Protein
 CD19
Synonyms
 CD19; B4; CVID3; Leu-12; MGC12802;Leu12
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Glu21-Lys291
Molecular Weight
 The protein has a predicted MW of 36 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human CD19, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Coltuximab, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.64 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His-Avi

Fluorophore

 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human CD19, His Tag with an affinity constant of 0.64 nM as determined in SPR assay (Biacore T200). Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human CD19, His Tag with an affinity constant of 0.64 nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human CD19, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Coltuximab, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human CD19, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Coltuximab, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human CD19, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for FMC63, mFc Tag with the EC50 of 11.4ng/ml determined by ELISA. Immobilized Biotinylated Human CD19, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for FMC63, mFc Tag with the EC50 of 11.4ng/ml determined by ELISA.
Biotinylated Human CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Additional Information
Learn more about our CD19 products Product Brochure: CD19 Antigens
Other Available Proteins:
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