Product Description |
Recombinant Biotinylated Human CD19 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Pro20-Lys291. |
Product Background |
CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores. |
Product Category |
Recombinant Protein |
Target |
CD19 |
Synonyms |
CD19; B4; CVID3; Leu-12; MGC12802 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Pro20-Lys291 |
Molecular Weight |
The protein has a predicted MW of 36 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human CD19, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Coltuximab, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.64 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human CD19, His Tag with an affinity constant of 0.64 nM as determined in SPR assay (Biacore T200). |
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Immobilized Biotinylated Human CD19, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Coltuximab, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). |
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Immobilized Biotinylated Human CD19, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for FMC63, mFc Tag with the EC50 of 11.4ng/ml determined by ELISA. |
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Biotinylated Human CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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