Biotinylated Human CD19 Protein (CD1-HMR102)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: >95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human CD19, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Coltuximab, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.64 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Pro20-Lys291
Catalog #CD1-HMR102
Regular price $600.00
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Product Description

Recombinant Biotinylated Human CD19 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Pro20-Lys291.

Product Background

CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores.

Product Category

Recombinant Protein

Protein

CD19

Synonyms

CD19; B4; CVID3; Leu-12; MGC12802

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Pro20-Lys291

Molecular Weight

The protein has a predicted MW of 36 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human CD19, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Coltuximab, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.64 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

>95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human CD19, His Tag with an affinity constant of 0.64 nM as determined in SPR assay (Biacore T200). Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human CD19, His Tag with an affinity constant of 0.64 nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human CD19, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Coltuximab, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human CD19, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Coltuximab, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human CD19, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for FMC63, mFc Tag with the EC50 of 11.4ng/ml determined by ELISA. Immobilized Biotinylated Human CD19, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for FMC63, mFc Tag with the EC50 of 11.4ng/ml determined by ELISA.
Biotinylated Human CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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