Name: Biotinylated Human CD19 Protein (CD1-HMR102)
Brand: KactusBio
SKU: CD1-HMR102-100UG
Price: 660.0 USD
Availability: InStock

Biotinylated Human CD19 Protein (CD1-HMR102)

Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human CD19, His Tag with an affinity constant of 0.64 nM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human CD19, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Coltuximab, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test).

Immobilized Biotinylated Human CD19, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for FMC63, mFc Tag with the EC50 of 11.4ng/ml determined by ELISA.

Biotinylated Human CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: C-His-Avi
Purity: >95% as determined by Bis-Tris PAGE
Activity: Immobilized Biotinylated Human CD19, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Coltuximab, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.64 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Pro20-Lys291

Catalog #CD1-HMR102

Size

100UG

500UG

Order now, ships by:

[{"id":46238158160094,"title":"100UG","option1":"100UG","option2":null,"option3":null,"sku":"CD1-HMR102-100UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Biotinylated Human CD19 Protein (CD1-HMR102) - 100UG","public_title":"100UG","options":["100UG"],"price":66000,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":46191218327774,"title":"500UG","option1":"500UG","option2":null,"option3":null,"sku":"CD1-HMR102-500UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Biotinylated Human CD19 Protein (CD1-HMR102) - 500UG","public_title":"500UG","options":["500UG"],"price":220000,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}}]

Product Description	Recombinant Biotinylated Human CD19 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Pro20-Lys291.
Product Background	CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	CD19
Synonyms	CD19; B4; CVID3; Leu-12; MGC12802
Accession	P15391-1
Species	Human
Biotinylated	yes
Amino Acid Range	Pro20-Lys291
Molecular Weight	The protein has a predicted MW of 36 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD19, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Coltuximab, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.64 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	>95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human CD19, His Tag with an affinity constant of 0.64 nM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human CD19, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Coltuximab, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test).
	Immobilized Biotinylated Human CD19, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for FMC63, mFc Tag with the EC50 of 11.4ng/ml determined by ELISA.
	Biotinylated Human CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.