Biotinylated Human FAP Protein (FAP-HM401B)

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Biotinylated: yes
Activity: Immobilized Biotinylated Human FAP, His Tag at 2ug/ml (100ul/Well) on streptavidin(5ug/ml) precoated plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Leu26-Asp760

Catalog: FAP-HM401B

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Product Description	Recombinant Biotinylated Human FAP Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Leu26-Asp760.
Product Background	Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.
Product Category	Recombinant Protein

Target	FAP
Synonyms	FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
Gene/Protein ID	Q12884
Species	Human
Exact Sequence	Leu26-Asp760
Molecular Weight	The protein has a predicted MW of 87.9 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Biotinylated Human FAP, His Tag at 2ug/ml (100ul/Well) on streptavidin(5ug/ml) precoated plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Biotinylated Human FAP, His Tag at 2ug/ml (100ul/Well) on streptavidin(5ug/ml) precoated plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test).
	Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >4000 pmol/min/µg (QC Test).
	The purity of Biotinylated Human FAP is greater than 95% as determined by SEC-HPLC.
	Biotinylated Human FAP on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.