Biotinylated Human FAP Protein (FAP-HM401B)
Catalog: FAP-HM401B
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Product Description | Recombinant Biotinylated Human FAP Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Leu26-Asp760. |
Product Background |
Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells. |
Product Category |
Recombinant Protein |
Target |
FAP |
Synonyms | FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA |
Gene/Protein ID |
Q12884 |
Species |
Human |
Exact Sequence |
Leu26-Asp760 |
Molecular Weight | The protein has a predicted MW of 87.9 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Biotinylated Human FAP, His Tag at 2ug/ml (100ul/Well) on streptavidin(5ug/ml) precoated plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-His-Avi |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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