Product Description |
Recombinant Biotinylated Human FAP Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Leu26-Asp760. |
Product Background |
Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Protein |
FAP |
Synonyms |
FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Leu26-Asp760 |
Molecular Weight |
The protein has a predicted MW of 87.10 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human FAP, His Tag at 2ug/ml (100ul/Well) on streptavidin(5ug/ml) precoated plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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|
Immobilized Biotinylated Human FAP, His Tag at 2ug/ml (100ul/Well) on streptavidin(5ug/ml) precoated plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA (QC Test). |
|
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Biotinylated Human FAP on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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The purity of Biotinylated Human FAP is greater than 95% as determined by SEC-HPLC. |
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