Biotinylated Human FAP Protein (FAP-HM401B)

  • Express System: Expi293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-FAP Antibody at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FAP, His Tag with the EC50 of 0.14μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu26-Asp760

Catalog: FAP-HM401B

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Product Description Recombinant Biotinylated Human FAP Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.It contains Leu26-Asp760.
Product Category Recombinant Protein
Target FAP
Synonyms FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
Gene/Protein ID Q12884
Species Human
Exact Sequence Leu26-Asp760
Molecular Weight The protein has a predicted MW of 87.9 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-FAP Antibody at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FAP, His Tag with the EC50 of 0.14μg/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 20mM Tirs, 150mM NaCl, 200mM L-Arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-FAP Antibody at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FAP, His Tag with the EC50 of 0.14μg/ml determined by ELISA.
The purity of Biotinylated Human FAP is greater than 95% as determined by SEC-HPLC.
Biotinylated Human FAP on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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