GMP Human IL-15 (GMP-CKS-HE015)
GMP Human IL-15 (GMP-CKS-HE015)
GMP Human IL-15 (GMP-CKS-HE015)
GMP Human IL-15 (GMP-CKS-HE015)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: ≥ 95% as determined by SEC-HPLC.
  • Amino Acid Range: Asn49-Ser162
Catalog #GMP-CKS-HE015
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GMP Human IL-15 (GMP-CKS-HE015) Summary

Product Description

 Recombinant GMP Human IL-15 is expressed from E.coli without tag.It contains Asn49-Ser162.

Product Background

 IL-15 belongs to the common gamma chain cytokine family and has pleiotropic immunological functions, and the protein is mainly produced by monocytes, dendritic cells, and epithelial cells. IL-15 plays a crucial role in the development, homeostasis, and function of T, NK and NK-T cells, and is additionally required for various functions of the B cells, dendritic cells (DC), macrophages, and mast cells. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.

Product Category

 Recombinant Protein / Cell therapy / Cell culture / Cytokines

GMP Human IL-15 (GMP-CKS-HE015) Specifications/Details

Protein
 IL-15
Synonyms
 Interleukin-15; IL-15; IL15; il 15; MGC9721
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Asn49-Ser162
Molecular Weight
 The protein has a predicted MW of 12.8 kDa same as Bis-Tris PAGE result.
Product Tag
 No Tag

Fluorophore

 Unconjugated
Expression System
 E.coli
Purity
 ≥ 95% as determined by SEC-HPLC.
Endotoxin
 ≤ 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 20 mM-Tris, 150 mM-NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Stability And Storage
 The product is shipped with ice bag. Store it immediately at -20°C±5°C upon arrival. ；-20°C±5°C for 36 months in lyophilized state. ；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

GMP Human IL-15 on Bis-Tris PAGE under reduced condition. GMP Human IL-15 on Bis-Tris PAGE under reduced condition.
Measured in a cell proliferation assay using CTLL-2 cells. The ED50-for this effect is 0.1 - 0.3 ng/mL. The specific activity of GMP Human IL-15 is > 1 × 10^7-IU/mg, which is calibrated against the human IL-15 WHO reference standard (NIBSC code: 95/554) (QC Test). Measured in a cell proliferation assay using CTLL-2 cells. The ED50-for this effect is 0.1 - 0.3 ng/mL. The specific activity of GMP Human IL-15 is > 1 × 10^7-IU/mg, which is calibrated against the human IL-15 WHO reference standard (NIBSC code: 95/554) (QC Test).
GMP Human IL-15 (reconstituted protein) was stored at 37°C, the protein is stable for 7 days determined by cell based assay. GMP Human IL-15 (reconstituted protein) was stored at 37°C, the protein is stable for 7 days determined by cell based assay.
GMP Human IL-15 (reconstituted protein) was stored at 4°C, the protein is stable for 90 days determined by cell based assay. GMP Human IL-15 (reconstituted protein) was stored at 4°C, the protein is stable for 90 days determined by cell based assay.
GMP Human IL-15 (reconstituted protein) was repeatedly frozen at -80°C and thawed at 37°C, the protein is stable after freezing and thawing 3 cycles determined by cell based assay. GMP Human IL-15 (reconstituted protein) was repeatedly frozen at -80°C and thawed at 37°C, the protein is stable after freezing and thawing 3 cycles determined by cell based assay.
Other Available Proteins:
Finding available proteins...
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