Product Description |
Recombinant GMP Human IL-15 is expressed from E.coli without tag.It contains Asn49-Ser162. |
Product Background |
IL-15 belongs to the common gamma chain cytokine family and has pleiotropic immunological functions, and the protein is mainly produced by monocytes, dendritic cells, and epithelial cells. IL-15 plays a crucial role in the development, homeostasis, and function of T, NK and NK-T cells, and is additionally required for various functions of the B cells, dendritic cells (DC), macrophages, and mast cells. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA. |
Product Category |
Recombinant Protein |
Target |
IL-15 |
Synonyms |
Interleukin-15; IL-15; IL15; il 15; MGC9721 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asn49-Ser162 |
Molecular Weight |
The protein has a predicted MW of 12.8 kDa same as Bis-Tris PAGE result. |
Product Tag |
No Tag |
Express System |
E.coli |
Purity |
≥ 95% as determined by SEC-HPLC. |
Endotoxin |
≤ 0.01 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20 mM-Tris, 150 mM-NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Measured in a cell proliferation assay using CTLL-2 cells. The ED50-for this effect is 0.1 - 0.3 ng/mL. The specific activity of GMP Human IL-15 is > 1 × 10^7-IU/mg, which is calibrated against the human IL-15 WHO reference standard (NIBSC code: 95/554) (QC Test). |
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GMP Human IL-15 (reconstituted protein) was stored at 37°C, the protein is stable for 7 days determined by cell based assay. |
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GMP Human IL-15 (reconstituted protein) was repeatedly frozen at -80°C and thawed at 37°C, the protein is stable after freezing and thawing 3 cycles determined by cell based assay. |
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GMP Human IL-15 on Bis-Tris PAGE under reduced condition. |
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GMP Human IL-15 (reconstituted protein) was stored at 4°C, the protein is stable for 90 days determined by cell based assay. |
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