Name: Human GDF-2/BMP-9 Protein (GDF-HM002)
Brand: KactusBio
SKU: GDF-HM002-100UG
Price: 429.0 USD
Availability: InStock

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test). The affinity constant of 26.57 pM as determined in SPR assay (Biacore T200).
Amino Acid Range: Ser320-Arg429

Catalog #GDF-HM002

Size

100UG

500UG

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Human GDF-2/BMP-9 Protein (GDF-HM002) Summary

Product Description	Recombinant Human GDF-2/BMP-9 Protein is expressed from HEK293 without tag.It contains Ser320-Arg429.
Product Background	Bone morphogenetic proteins (BMPs) are expressed in different cell types of the human ovarian follicle and play important roles in the regulation of ovarian function. BMP-9, also known as growth differentiation factor-2 (GDF-2), belongs to the transforming growth factor-beta (TGF-β) superfamily. BMP-9 is mainly synthesized in the liver and secreted into the blood which allows it to regulate various physiological and pathological functions.
Product Category	Recombinant Protein / Cell therapy / Cell culture / Cytokines

Human GDF-2/BMP-9 Protein (GDF-HM002) Specifications/Details

Protein	GDF-2/BMP-9
Synonyms	GDF-2; GDF2; BMP-9; BMP9
Accession	Q9UK05
Species	Human
Biotinylated	no
Amino Acid Range	Ser320-Arg429
Molecular Weight	The protein has a predicted MW of 12.08 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test). The affinity constant of 26.57 pM as determined in SPR assay (Biacore T200).
Product Tag	No Tag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 4mM HCL. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 4mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human/Cynomolgus Activin RIIB, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF-2, No Tag with an affinity constant of 26.57 pM as determined in SPR assay (Biacore T200).
	Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test).
	Immobilized Human GDF-2, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GDF-2 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA.
	Human GDF-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50-for this effect is 0.4-4 ng/mL (QC Test).