ELISA binding curve showing the interaction between Human SCF (no tag) and Human CD117 (His tag). The graph displays a dose-dependent increase in OD450, reaching saturation near 3.3.
Human SCF Protein (SCF-HE001)
Human SCF Protein (SCF-HE001)
Human SCF Protein (SCF-HE001)
Human SCF Protein (SCF-HE001)
Human SCF Protein (SCF-HE001)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human SCF at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Glu26-Ala189
Catalog #SCF-HE001
Regular price $223.30
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Product Description

Recombinant Human SCF Protein is expressed from E.coli without tag.It contains Glu26-Ala189.

Product Background

Kit-ligand (Kitl) also known as steel factor, stem cell factor and mast cell growth factor plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. Kitl exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes.

Product Category

Recombinant Protein / Cell therapy / Cell culture / Cytokines
Protein
SCF
Synonyms
MGF; c-Kit ligand; sKITLG; KITLG; FPH2; KL-1; Kitl; SF; SHEP7; KL
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Glu26-Ala189
Molecular Weight
The protein has a predicted MW of 18.45 kDa same as Bis-Tris PAGE result.
Activity
Immobilized Human SCF at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

No Tag
Expression System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 0.02 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human SCF at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA (QC Test). Immobilized Human SCF at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA (QC Test).
Human SCF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human SCF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using Mo7e cells. The ED50-for this effect is 1-8 ng/mL (QC Test). Measured in a cell proliferation assay using Mo7e cells. The ED50-for this effect is 1-8 ng/mL (QC Test).
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