Human FGF8b-Protein (FGF-HE08B)
Human FGF8b-Protein (FGF-HE08B)
Human FGF8b-Protein (FGF-HE08B)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Amino Acid Range: Gln23-Arg215
Catalog #FGF-HE08B
Regular price $330.00
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Product Description

Recombinant Human FGF8b-Protein is expressed from E.coli without tag.It contains Gln23-Arg215.

Product Background

Two of the four human FGF8 splice isoforms, FGF8a and FGF8b, are expressed in the mid-hindbrain region during development. Although the only difference between these isoforms is the presence of an additional 11 amino acids at the N terminus of FGF8b, these isoforms possess remarkably different abilities to pattern the midbrain and anterior hindbrain.

Product Category

Recombinant Protein / Cell therapy / Cell culture / Cytokines
Protein
FGF8b
Synonyms
FGF8b; FGF-8b; FGF-8; FGF8; FGF 8; AIGF; HBGF-8; HBGF8; HBGF 8;
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln23-Arg215
Molecular Weight
The protein has a predicted MW of 22.39 kDa same as Bis-Tris PAGE result.

Product Tag

No Tag
Expression System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 0.01 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 20mM Tris, 300mM NaCl (pH 8.5). Normally 8% mannitol or 15% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human FGF8b-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human FGF8b-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblasts. The ED50-for this effect is 5-20 ng/mL. Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblasts. The ED50-for this effect is 5-20 ng/mL.
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