Product Description |
Recombinant Human FGF8b-Protein is expressed from E.coli without tag.It contains Gln23-Arg215. |
Product Background |
Two of the four human FGF8 splice isoforms, FGF8a and FGF8b, are expressed in the mid-hindbrain region during development. Although the only difference between these isoforms is the presence of an additional 11 amino acids at the N terminus of FGF8b, these isoforms possess remarkably different abilities to pattern the midbrain and anterior hindbrain. |
Product Category |
Recombinant Protein |
Target |
FGF8b |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln23-Arg215 |
Molecular Weight |
The protein has a predicted MW of 22.39 kDa same as Bis-Tris PAGE result. |
Product Tag |
No Tag |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20mM Tris, 300mM NaCl (pH 8.5). Normally 8% mannitol or 15% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human FGF8b-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblasts. The ED50-for this effect is 5-20 ng/mL. |
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