Human EPO/Erythropoietin-Protein (EPO-HM001)
Human EPO/Erythropoietin-Protein (EPO-HM001)
Human EPO/Erythropoietin-Protein (EPO-HM001)
Human EPO/Erythropoietin-Protein (EPO-HM001)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human EPO, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala28-Arg193
Catalog #EPO-HM001
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Product Description
 Recombinant Human EPO/Erythropoietin-Protein is expressed from HEK293 without tag.It contains Ala28-Arg193.
Product Background
 Erythropoietin (EPO) is a circulating hormone conventionally considered to be responsible for erythropoiesis. In addition to facilitating red blood cell production, EPO has pluripotent potential, such as for cognition improvement, neurogenesis, and anti-fibrotic, anti-apoptotic, anti-oxidative, and anti-inflammatory effects.
Product Category
 Recombinant Protein
Target
 EPO/Erythropoietin
Synonyms
 EPO; Erythropoietin; EP; MVCD2; Epoetin
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala28-Arg193
Molecular Weight
 The protein has a predicted MW of 18.39 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human EPO, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA. See testing image for detail.
Product Tag
 No Tag
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human EPO, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA.
Human EPO on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50-for this effect is 0.4 ‑ 1 ng/mL (QC Test).
The purity of Human EPO is greater than 95% as determined by SEC-HPLC.

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