Human EPO/Erythropoietin-Protein (EPO-HM001)
Human EPO/Erythropoietin-Protein (EPO-HM001)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human EPO, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala28-Arg193
Catalog #EPO-HM001
Regular price $180.00
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Product Description

Recombinant Human EPO/Erythropoietin-Protein is expressed from HEK293 without tag.It contains Ala28-Arg193.

Product Background

Erythropoietin (EPO) is a circulating hormone conventionally considered to be responsible for erythropoiesis. In addition to facilitating red blood cell production, EPO has pluripotent potential, such as for cognition improvement, neurogenesis, and anti-fibrotic, anti-apoptotic, anti-oxidative, and anti-inflammatory effects.

Product Category

Recombinant Protein

Protein

EPO/Erythropoietin

Synonyms

EPO; Erythropoietin; EP; MVCD2; Epoetin

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Ala28-Arg193

Molecular Weight

The protein has a predicted MW of 18.39 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human EPO, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

No Tag

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 0.1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human EPO, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA. Immobilized Human EPO, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA.
Human EPO on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human EPO on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50-for this effect is 0.4 ‑ 1 ng/mL (QC Test). Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50-for this effect is 0.4 ‑ 1 ng/mL (QC Test).
The purity of Human EPO is greater than 95% as determined by SEC-HPLC. The purity of Human EPO is greater than 95% as determined by SEC-HPLC.

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