Product Description |
Recombinant Human EPO/Erythropoietin-Protein is expressed from HEK293 without tag.It contains Ala28-Arg193. |
Product Background |
Erythropoietin (EPO) is a circulating hormone conventionally considered to be responsible for erythropoiesis. In addition to facilitating red blood cell production, EPO has pluripotent potential, such as for cognition improvement, neurogenesis, and anti-fibrotic, anti-apoptotic, anti-oxidative, and anti-inflammatory effects. |
Product Category |
Recombinant Protein / Cell therapy / Cell culture / Cytokines |
Protein |
EPO/Erythropoietin |
Synonyms |
EPO; Erythropoietin; EP; MVCD2; Epoetin |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala28-Arg193 |
Molecular Weight |
The protein has a predicted MW of 18.39 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human EPO, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
No Tag |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Immobilized Human EPO, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA. |
|
|
Human EPO on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50-for this effect is 0.4 ‑ 1 ng/mL (QC Test). |
|
|
The purity of Human EPO is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
More from Cell Therapy Proteins
Recently viewed
