Human IL-13 Protein (IL1-HE013)
Human IL-13 Protein (IL1-HE013)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-13, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 3.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly21-Asn132
Catalog #IL1-HE013
Regular price $375.00
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Human IL-13 Protein (IL1-HE013) Summary

Product Description

Recombinant Human IL-13 Protein is expressed from E.coli without tag.It contains Gly21-Asn132.

Product Background

Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).

Product Category

Recombinant Protein / Cell therapy / Cell culture / Cytokines

Human IL-13 Protein (IL1-HE013) Specifications/Details

Protein
IL-13
Synonyms
IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gly21-Asn132
Molecular Weight
The protein has a predicted MW of 12.34 kDa same as Bis-Tris PAGE result.
Activity
Immobilized Human IL-13, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 3.6ng/ml determined by ELISA. See testing image for detail.
Product Tag
No Tag
Expression System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-13, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 3.6ng/ml determined by ELISA. Immobilized Human IL-13, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 3.6ng/ml determined by ELISA.
Human IL-13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50-for this effect is typically 0.5-3 ng/mL. Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50-for this effect is typically 0.5-3 ng/mL.
Loaded Human IL-13Ra2, His Tag on Anti-His-Biosensor can bind Human IL-13, No Tag with an affinity constant of 0.63 nM as determined in BLI assay (Gator® Prime). Loaded Human IL-13Ra2, His Tag on Anti-His-Biosensor can bind Human IL-13, No Tag with an affinity constant of 0.63 nM as determined in BLI assay (Gator® Prime).
The purity of Human IL-13 is greater than 95% as determined by SEC-HPLC. The purity of Human IL-13 is greater than 95% as determined by SEC-HPLC.
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