Name: Human FGF1 Protein (FGF-HE00A)
Brand: KactusBio
SKU: FGF-HE00A-100UG
Price: 107.8 USD
Availability: InStock

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 42.45 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Phe16-Asp155

Catalog #FGF-HE00A

Size

100UG

500UG

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Human FGF1 Protein (FGF-HE00A) Summary

Product Description	Recombinant Human FGF1 Protein is expressed from E.coli without tag.It contains Phe16-Asp155.
Product Background	Fibroblast Growth Factor 1 (Fgf1), also known as acidic FGF (aFGF), is involved in the regulation of various biological processes, ranging from development to disease pathogenesis. It is a single chain polypeptide and is highly expressed in adult brain and kidney tissues. Its expression has been shown to be directed by multiple tissue-specific promoters, which generate transcripts of varying lengths.
Product Category	Recombinant Protein / Cell therapy / Cell culture / Cytokines

Human FGF1 Protein (FGF-HE00A) Specifications/Details

Protein	FGF1
Synonyms	FGF-1; aFGF; ECGF; HBGF-1; FGFA
Accession	P05230-1
Species	Human
Biotinylated	no
Amino Acid Range	Phe16-Asp155
Molecular Weight	The protein has a predicted MW of 15.83 kDa same as Bis-Tris PAGE result.
Activity	The affinity constant of 42.45 nM as determined in SPR assay (Biacore T200).
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human FGFR1 alpha (IIIc), His Tag captured on CM5 Chip via Anti-His Antibody can bind Human FGF1, No tag with an affinity constant of 42.45 nM as determined in SPR assay (Biacore T200).
	Human FGF1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50-for this effect is typically 0.1-0.7 ng/mL (QC Test).
	The purity of Human FGF1 is greater than 95% as determined by SEC-HPLC.