Product Description |
Recombinant GMP Human IL-7 is expressed from E.coli without tag.It contains Asp26-His177. |
Product Background |
Interleukin (IL)-7 is a non-redundant cytokine in T-cell development and function. It is required in early T-cell development as well as for T-cell homeostasis and is secreted by stromal cells in the thymic and bone marrow (BM) environment. IL-7 binds to its receptor which is composed of the two chains IL-7Rα (CD127), shared with the thymic stromal lymphopoietin (TSLP), and the common γ chain (CD132) for IL-2, IL-15, IL-9 and IL-21. Whereas γc is expressed by most hematopoietic cells, IL-7Rα is nearly exclusively expressed on lymphoid cells. After binding to its receptor, IL-7 signals through two different pathways: Jak-Stat (Janus kinase-Signal transducer and activator of transcription) and PI3K/Akt responsible for differentiation and survival, respectively. |
Product Category |
Recombinant Protein / Cell therapy / Cell culture / Cytokines |
Protein |
IL-7 |
Synonyms |
IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp26-His177 |
Molecular Weight |
The protein has a predicted MW of 17.5 kDa same as Bis-Tris PAGE result. |
Product Tag |
No Tag |
Expression System |
E.coli |
Purity |
≥ 95% as determined by SEC-HPLC. |
Endotoxin |
≤ 0.01 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
The product is shipped with ice bag. Store it immediately at -20°C±5°C upon arrival. ；-20°C±5°C for 36 months in lyophilized state. ；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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GMP Human IL-7 on Bis-Tris PAGE under reduced condition. |
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Measured in a cell proliferation assay using PHA-activated human peripheral blood mononuclear cell (PBMC). The specific activity of GMP Human IL-7 is > 1 × 10^8-IU/mg, which is calibrated against the human IL-7 WHO reference standard (NIBSC code: 90/530) (QC Test). |
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