GMP Human IL-7 (GMP-CKS-HE007)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: ≥ 95% as determined by SEC-HPLC.
  • Amino Acid Range: Asp26-His177
Catalog #GMP-CKS-HE007
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Product Description
 Recombinant GMP Human IL-7 is expressed from E.coli without tag.It contains Asp26-His177.
Product Background
 Interleukin (IL)-7 is a non-redundant cytokine in T-cell development and function. It is required in early T-cell development as well as for T-cell homeostasis and is secreted by stromal cells in the thymic and bone marrow (BM) environment. IL-7 binds to its receptor which is composed of the two chains IL-7Rα (CD127), shared with the thymic stromal lymphopoietin (TSLP), and the common γ chain (CD132) for IL-2, IL-15, IL-9 and IL-21. Whereas γc is expressed by most hematopoietic cells, IL-7Rα is nearly exclusively expressed on lymphoid cells. After binding to its receptor, IL-7 signals through two different pathways: Jak-Stat (Janus kinase-Signal transducer and activator of transcription) and PI3K/Akt responsible for differentiation and survival, respectively.
Product Category
 Recombinant Protein
Target
 IL-7
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Asp26-His177
Molecular Weight
 The protein has a predicted MW of 17.5 kDa same as Bis-Tris PAGE result.
Product Tag
 No Tag
Express System
 E.coli
Purity
 ≥ 95% as determined by SEC-HPLC.
Endotoxin
 ≤ 0.01 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Measured in a cell proliferation assay using PHA-activated human peripheral blood mononuclear cell (PBMC). The specific activity of GMP Human IL-7 is > 1 × 10^8-IU/mg, which is calibrated against the human IL-7 WHO reference standard (NIBSC code: 90/530) (QC Test).
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