Human IL-13 (R130Q) Protein (IL1-HE03M)

Human IL-13Ra1, hFc Tag (Cat. ILR-HM2R1) captured on CM5 Chip via ProteinA can bind Human IL-13 (R130Q), No Tag with an affinity constant of 10.65 nM as determined in SPR assay (Biacore T200).

Immobilized Human IL-13 (R130Q), No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 38.3ng/ml determined by ELISA.

Human IL-13 (R130Q) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-3 ng/mL.

The purity of Human IL-13 (R130Q) is greater than 95% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-13 (R130Q), No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 38.3ng/ml determined by ELISA. The affinity constant of 10.65 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gly21-Asn132(R130Q)

Catalog #IL1-HE03M

Price

$345⁰⁰

Size

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Human IL-13 (R130Q) Protein (IL1-HE03M) Summary

Product Description

Recombinant Human IL-13 (R130Q) Protein is expressed from E.coli without tag.It contains Gly21-Asn132 (R130Q).

Product Background

Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).

Human IL-13 (R130Q) Protein (IL1-HE03M) Specifications/Details

Protein	IL-13
Synonyms	IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789;Interleukin-13; IL 13
Accession	AAK53823
Species	Human
Biotinylated	no
Amino Acid Range	Gly21-Asn132(R130Q)
Molecular Weight	The protein has a predicted MW of 12.44 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human IL-13 (R130Q), No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 38.3ng/ml determined by ELISA. The affinity constant of 10.65 nM as determined in SPR assay (Biacore T200).
Product Tag	No Tag
Fluorophore	Unconjugated
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4).
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human IL-13Ra1, hFc Tag (Cat. ILR-HM2R1) captured on CM5 Chip via ProteinA can bind Human IL-13 (R130Q), No Tag with an affinity constant of 10.65 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-13 (R130Q), No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 38.3ng/ml determined by ELISA.
	Human IL-13 (R130Q) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-3 ng/mL.
	The purity of Human IL-13 (R130Q) is greater than 95% as determined by SEC-HPLC.