Mouse TPO/Thrombopoietin Protein (TPO-MM102)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Ser22-Thr356
Catalog #TPO-MM102
Regular price $270.00
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Product Description

Recombinant Mouse TPO/Thrombopoietin Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser22-Thr356.

Product Background

Thrombopoietin (TPO), the c-Mpl ligand, is produced constitutively in liver and other organs, circulates in the bloodstream, and is delivered to bone marrow, where it stimulates the early development of multiple hematopoietic lineages and megakaryocytopoiesis. The concentration of TPO in blood is regulated by c-Mpl mass on platelets and megakaryocytes.

Product Category

Recombinant Protein

Protein

TPO/Thrombopoietin

Synonyms

Thrombopoietin; TPO; MGDF; THPO; ML; C-mpl ligand

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Ser22-Thr356

Molecular Weight

The protein has a predicted MW of 37.20 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Bis-Tris PAGE result.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse TPO on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse TPO on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.4-2.4 ng/ml (QC Test). Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.4-2.4 ng/ml (QC Test).
The purity of Mouse TPO is greater than 95% as determined by SEC-HPLC. The purity of Mouse TPO is greater than 95% as determined by SEC-HPLC.

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