Biotinylated Human Activin RIIA/ACVR2A Protein (ARA-HM42AB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human Activin A, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Activin RIIA, His Tag with the EC50 of 0.48 ug/ml determined by ELISA (QC Test). The affinity constant of 0.10 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala20-Pro135
Catalog #ARA-HM42AB
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Product Description
 Recombinant Biotinylated Human Activin RIIA/ACVR2A Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ala20-Pro135.
Product Background
 Activin A receptor type 2A (ACVR2A) is a membrane receptor in the transforming growth factor- beta (TGF-β signaling pathway, which is involved in the regulation of cell proliferation, migration, and apoptosis. Loss of ACVR2A has an important role in cancer progression and distant metastasis and may serve as a prognostic marker in patients with colon cancer.
Product Category
 Recombinant Protein
Target
 Activin RIIA/ACVR2A
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ala20-Pro135
Molecular Weight
 The protein has a predicted MW of 16.31 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Activin A, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Activin RIIA, His Tag with the EC50 of 0.48 ug/ml determined by ELISA (QC Test). The affinity constant of 0.10 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Activin RIIA, His Tag immobilized on CM5 Chip can bind Human Activin A, No Tag with an affinity constant of 0.10 nM as determined in SPR assay (Biacore T200).
Immobilized Human Activin A, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Activin RIIA, His Tag with the EC50 of 0.48 ug/ml determined by ELISA (QC Test).
Biotinylated Human Activin RIIA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Activin RIIA is greater than 95% as determined by SEC-HPLC.

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