Product Description |
Recombinant Biotinylated Human/Cynomolgus Activin RIIA/ACVR2A Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ala20-Pro135. |
Product Background |
Activin A receptor type 2A (ACVR2A) is a membrane receptor in the transforming growth factor- beta (TGF-β signaling pathway, which is involved in the regulation of cell proliferation, migration, and apoptosis. Loss of ACVR2A has an important role in cancer progression and distant metastasis and may serve as a prognostic marker in patients with colon cancer. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
Activin RIIA/ACVR2A |
Synonyms |
ACTR-IIA; ACTRIIA; ACVR2A; ACVR2 |
Accession |
|
Species |
Human/Cynomolgus |
Biotinylated |
yes |
Amino Acid Range |
Ala20-Pro135 |
Molecular Weight |
The protein has a predicted MW of 16.31 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Activin A, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus Activin RIIA, His Tag with the EC50 of 0.48 ug/ml determined by ELISA (QC Test). The affinity constant of 0.10 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Biotinylated Human/Cynomolgus Activin RIIA, His Tag immobilized on CM5 Chip can bind Human Activin A, No Tag with an affinity constant of 0.10 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human Activin A, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus Activin RIIA, His Tag with the EC50 of 0.48 ug/ml determined by ELISA (QC Test). |
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Biotinylated Human/Cynomolgus Activin RIIA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human/Cynomolgus Activin RIIA is greater than 95% as determined by SEC-HPLC. |
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