• Express System: E.coli
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human Activin E, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag (Cat. ARA-HM22A) with the EC50 of 0.95ug/ml determined by ELISA.
  • Amino Acid Range: Thr237-Ser350
Catalog #IHB-HE10E
Price
Regular price $45000
Regular price Sale price $45000
/
Size
Avantor VWR Fisher Scientific

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Human Activin E Protein (IHB-HE10E) Summary

Product Description

Recombinant Human Activin E Protein is expressed from E.coli with His tag at the N-terminus.It contains Thr237-Ser350.

Product Background

Activin E is a liver-derived hepatokine belonging to the transforming growth factor-β superfamily, and it promotes energy expenditure by activating brown and beige adipocytes. Activin E also possesses anti-lipolytic activity.

Human Activin E Protein (IHB-HE10E) Specifications/Details

Protein
Activin E
Synonyms
Activin beta-E chain; EGM_03412; Inhibin subunit beta E; INHBE
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Thr237-Ser350
Molecular Weight
The protein has a predicted MW of 13.56 kDa. The protein migrates to 15-18 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Activin E, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag (Cat. ARA-HM22A) with the EC50 of 0.95ug/ml determined by ELISA.
Product Tag
N-His
Fluorophore
Unconjugated
Expression System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in 4mM HCl.
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Activin E, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag (Cat. ARA-HM22A) with the EC50 of 0.95ug/ml determined by ELISA. Immobilized Human Activin E, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag (Cat. ARA-HM22A) with the EC50 of 0.95ug/ml determined by ELISA.
Human Activin E on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%. Human Activin E on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
Response to human Activin E protein (FOLD). Measured by a reporter gene assay using Activin E/ALK7 Effector Reporter Cell. The maxinduction fold was approximately 370. Response to human Activin E protein (FOLD). Measured by a reporter gene assay using Activin E/ALK7 Effector Reporter Cell. The maxinduction fold was approximately 370.
Other Available Proteins:
Finding available proteins...