• Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human Activin B, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag (Cat. ARB-HM22B) with the EC50 of 10.8ng/ml determined by ELISA.
  • Amino Acid Range: Gly293-Ala407
Catalog #ACV-HM00B
Price
Regular price $41300
Regular price Sale price $41300
/
Size
Avantor VWR Fisher Scientific

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Human Activin B Protein (ACV-HM00B) Summary

Product Description

 Recombinant Human Activin B Protein is expressed from HEK293 without tag.It contains Gly293-Ala407.

Product Background

 The gene inhibin subunit beta B (INHBB), also named Activin B encodes the inhibin βB subunit, which is involved in forming protein members of the transforming growth factor-β (TGF-β) superfamily. The TGF-β superfamily is extensively involved in cell proliferation, differentiation, adhesion, movement, metabolism, communication, and death.

Human Activin B Protein (ACV-HM00B) Specifications/Details

Protein
 Activin B
Synonyms
 INHBB; Activin B; Inhibin beta B chain;
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly293-Ala407
Molecular Weight
 The protein has a predicted MW of 12.81 kDa. Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 24-30 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Activity
 Immobilized Human Activin B, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag (Cat. ARB-HM22B) with the EC50 of 10.8ng/ml determined by ELISA.
Product Tag
 No Tag
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 4mM HCL. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Activin B, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag (Cat. ARB-HM22B) with the EC50 of 10.8ng/ml determined by ELISA. Immobilized Human Activin B, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag (Cat. ARB-HM22B) with the EC50 of 10.8ng/ml determined by ELISA.
Immobilized Human Activin B, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag (Cat. ARA-HM22A) with the EC50 of 5.4ng/ml determined by ELISA. Immobilized Human Activin B, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag (Cat. ARA-HM22A) with the EC50 of 5.4ng/ml determined by ELISA.
Human Activin B Protein on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%. Human Activin B Protein on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 10 ng/mL. Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 10 ng/mL.
Loaded Human/Cynomolgus Activin RIIA, hFc Tag (Cat. ARA-HM22A) on ProA-Biosensor can bind Human Activin B, No Tag with an affinity constant of 0.25 nM as determined in BLI assay. Loaded Human/Cynomolgus Activin RIIA, hFc Tag (Cat. ARA-HM22A) on ProA-Biosensor can bind Human Activin B, No Tag with an affinity constant of 0.25 nM as determined in BLI assay.
Loaded Human/Cynomolgus Activin RIIB, hFc Tag (Cat. ARB-HM22B) on ProA-Biosensor can bind Human Activin B, No Tag with an affinity constant of 0.19 nM as determined in BLI assay. Loaded Human/Cynomolgus Activin RIIB, hFc Tag (Cat. ARB-HM22B) on ProA-Biosensor can bind Human Activin B, No Tag with an affinity constant of 0.19 nM as determined in BLI assay.
Other Available Proteins:
Finding available proteins...