BspQI (BSP-BE101)

  • Description: Type IIS restriction enzyme that recognizes asymmetric DNA sequences and cleaves outside of the recognition site
    Cleavage Site:
    5’...GCTCTTC(N)1↓...3’
    3’...CGAGAAG(N)4↑...5’
  • Applications: Molecular Cloning
    Restriction site mapping
    Genotyping
    SNP

Catalog: BSP-BE101

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Product Description BspQI is a Type IIS restriction enzyme that recognizes asymmetric DNA sequences and cleaves outside of the recognition site. BspQI completes cleavage within 15 to 30 min at 50°C. Recombinant Albumin was added to the 10X BspQI Reaction Buffer for stability and consistency. Isoschizomers for BspQI include SapI, PciSI and LguI.

Product Background

BspQI is a Type IIS restriction enzyme that recognizes asymmetric DNA sequences and cleaves outside of the recognition site. BspQI completes cleavage within 15 to 30 min at 50°C. Recombinant Albumin was added to the 10X BspQI Reaction Buffer for stability and consistency. Isoschizomers for BspQI include SapI, PciSI and LguI.
Synonyms BspQ1
Application Molecular Cloning
Restriction site mapping
Genotyping
SNP
Concentration 10U/μL
Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 50°C in a total reaction volume of 50 µL.
Source E. coli
Form Liquid
Stability And Storage Store at -20°C ± 5°C for up to 24 months. Avoid repeated freeze/thaw cycles. Transport on dry ice.
Restriction site for BspQI.

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