- Description: Type IIS restriction enzyme that recognizes asymmetric DNA sequences and cleaves outside of the recognition site
Cleavage Site:
5’...GCTCTTC(N)1↓...3’
3’...CGAGAAG(N)4↑...5’ - Applications: Molecular Cloning
Restriction site mapping
Genotyping
SNP
Product Description |
BspQI is a Type IIS restriction enzyme that recognizes asymmetric DNA sequences and cleaves outside of the recognition site. BspQI completes cleavage within 15 to 30 min at 50°C. Recombinant Albumin was added to the 10X BspQI Reaction Buffer for stability and consistency. Isoschizomers for BspQI include SapI, PciSI and LguI. |
Product Background |
BspQI is a Type IIS restriction enzyme that recognizes asymmetric DNA sequences and cleaves outside of the recognition site. BspQI completes cleavage within 15 to 30 min at 50°C. Recombinant Albumin was added to the 10X BspQI Reaction Buffer for stability and consistency. Isoschizomers for BspQI include SapI, PciSI and LguI. |
Synonyms |
BspQ1 |
Applications |
Molecular Cloning Restriction site mapping Genotyping SNP |
Concentration |
10U/μL |
Unit Definition |
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 50°C in a total reaction volume of 50 µL. |
Source |
E. coli |
Form |
Liquid |
Stability And Storage |
Store at -20°C ± 5°C for up to 24 months. Avoid repeated freeze/thaw cycles. Transport on dry ice. |
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