• Description: Type IIS restriction enzyme that recognizes asymmetric DNA sequences and cleaves outside of the recognition site
    Cleavage Site:
    5’...GCTCTTC(N)1↓...3’
    3’...CGAGAAG(N)4↑...5’
  • Applications: Molecular Cloning
    Restriction site mapping
    Genotyping
    SNP
Catalog #BSP-BE101
Price
Regular price $2750
Regular price Sale price $2750
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BspQI (BSP-BE101) Summary

Product Description

BspQI is a Type IIS restriction enzyme that recognizes asymmetric DNA sequences and cleaves outside of the recognition site. BspQI completes cleavage within 15 to 30 min at 50°C. Recombinant Albumin was added to the 10X BspQI Reaction Buffer for stability and consistency. Isoschizomers for BspQI include SapI, PciSI and LguI.

Product Background

BspQI is a Type IIS restriction enzyme that recognizes asymmetric DNA sequences and cleaves outside of the recognition site. BspQI completes cleavage within 15 to 30 min at 50°C. Recombinant Albumin was added to the 10X BspQI Reaction Buffer for stability and consistency. Isoschizomers for BspQI include SapI, PciSI and LguI.

BspQI (BSP-BE101) Specifications/Details

Synonyms
BspQ1
Applications
Molecular Cloning
Restriction site mapping
Genotyping
SNP
Concentration
10U/μL
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 50°C in a total reaction volume of 50 µL.
Source
E. coli

Shipping, Reconstitution, & Storage

Form
Liquid
Stability And Storage
Store at -20°C ± 5°C for up to 24 months. Avoid repeated freeze/thaw cycles. Transport on dry ice.

Validation Images

Restriction site for BspQI. Restriction site for BspQI.
Other Available Proteins:
Finding available proteins...