T7 RNA Polymerase, GMP grade (GMP-T7P-EE101)

Catalog: GMP-T7P-EE101


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Product Description T7 RNA Polymerase is the key enzyme for in vitro transcription of mRNA. It efficiently incorporates standard or modified nucleoside triphosphates (NTPs) into RNA transcripts. T7 RNA Polymerase initiates transcription at the G* in the classic T7 promoter-taatacgactcactataG*GG. When using GAG cap1-Analog for co-transcription, taatacgactcactataA*GG is recommended.

Restriction enzymes that produce blunt ends or 5'-overhangs are recommended.
The cofactor Mg2+ is required for the synthesis.
Application In vitro transcription, RNA analysis, Radiolabeled RNA probe preparation, Hybridization, RNase protection assay
Concentration 50U/µL
Unit Definition One unit is defined as the amount of enzyme required to incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50μl in 1 hour at 37°C.
Source E.coli strain that carries the T7 RNA Polymerase gene
Quality Standards Activity (Molecular Beacon): ≥ 50kU/mL
Purity (SEC-HPLC): ≥ 95%
Residual Endonuclease: Negative
Residual Exonuclease: Negative
Residual DNase: Negative
Residual RNase: Negative
Residual Protease: Negative
Endotoxin: ≤ 10EU/mL
Residual Host Cell DNA: ≤ 100pg/mL
Residual Host Protein: ≤ 20ng/mg
Residual Heavy Metal: ≤ 10ppm
Bioburden: ≤ 1CFU/10mL
Form Liquid
Shipping Shipped with blue ice
Stability And Storage -20±5°C for 24 months. Avoid repeated freeze-thaw cycles.
Figure 1. Three plasmids (lane 1 with poly A tail, lane 2 and 3 without poly A tail) were used as templates in a 2-hour reaction at 37 °C in a 20 μl system. The transcription length was 2000 nt, 4000 nt, and 2000 nt, respectively. The mRNA was not purified.
Figure 2. Detection of T7 RNA Polymerase by molecular beacon. The detection principle is that when the molecular beacon and transcript specifically bind, the conformation changes and the fluorescence intensity changes. Three batches of T7 RNA Polymerase were detected with a similar slope across all batches, indicating stable activity across batches.
Figure 3. Yield was assessed via nanodrop and Agilent 4150. Purity was assessed by CE. Overall, KACTUS T7 RNA Polymerase showed yield and purity comparable to those of leading competitors.

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