T7 RNA Polymerase, GMP Grade (GMP-T7P-EE101)
|Product Description||T7 RNA Polymerase is the key enzyme for in vitro transcription of mRNA. It efficiently incorporates standard or modified nucleoside triphosphates (NTPs) into RNA transcripts. Restriction enzymes that produce blunt ends or 5'-overhangs are recommended.
The cofactor Mg2+ is required for the synthesis.
Expressed in an E.coli strain that carries the T7 RNA Polymerase gene.
|Product Category||GMP Enzyme|
|Application||In vitro transcription(IVT)
Radiolabeled RNA probe preparation
RNase protection assay
|Unit Definition||One unit is defined as the amount of enzyme required to incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50μl in 1 hour at 37°C.|
|Molecular Weight||98 kD|
Manufactured to the GMP guidelines Antibiotic-free and animal-free production Suitable for manufacturing of mRNA therapeutics and vaccines
|QC Standard||Activity: >=50 kU/ml
Residual Endonuclease: Negative
Residual Exonuclease: Negative
Residual RNase: Negative
Residual Protease: Negative
Endotoxin: <=10 EU/ml
Residual Host Cell DNA: <=100 pg/MG
Residual Host Protein: <=50 ng/MG
Bioburden: <=1 CFU/10 ml
|Shipping||Shipped with blue ice|
|Stability And Storage||-20°C for 12 months. Avoid repeated freeze-thaw cycles.|