T7 RNA Polymerase, GMP Grade (GMP-T7P-EE101)

  • Quality Statement:
    Manufactured to the GMP guidelines

    Antibiotic-free and animal-free production

    Suitable for manufacturing of mRNA therapeutics and vaccines
  • Express System: E.coli

Catalog: GMP-T7P-EE101

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Product Description T7 RNA Polymerase is the key enzyme for in vitro transcription of mRNA. It efficiently incorporates standard or modified nucleoside triphosphates (NTPs) into RNA transcripts. Restriction enzymes that produce blunt ends or 5'-overhangs are recommended.
The cofactor Mg2+ is required for the synthesis.
Expressed in an E.coli strain that carries the T7 RNA Polymerase gene.
Product Category GMP Enzyme
Application In vitro transcription(IVT)
RNA analysis
Radiolabeled RNA probe preparation
Hybridization
RNase protection assay
Concentration 50 U/UL
Unit Definition One unit is defined as the amount of enzyme required to incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50μl in 1 hour at 37°C.
Molecular Weight 98 kD
Express System E.coli
Quality Statement
Manufactured to the GMP guidelines

Antibiotic-free and animal-free production

Suitable for manufacturing of mRNA therapeutics and vaccines
QC Standard Activity: >=50 kU/ml
Purity: >=95%
Residual Endonuclease: Negative
Residual Exonuclease: Negative
Residual RNase: Negative
Residual Protease: Negative
Endotoxin: <=10 EU/ml
Residual Host Cell DNA: <=100 pg/MG
Residual Host Protein: <=50 ng/MG
Bioburden: <=1 CFU/10 ml
Form Liquid
Shipping Shipped with blue ice
Stability And Storage -20°C for 12 months. Avoid repeated freeze-thaw cycles.
Fig. 1 Three plasmids (lane 1 with poly A tail, lane 2 and 3 without poly A tail) were used as templates, and the reaction was 2 h at 37 °C in the 20μl system, and the transcription length was 2000 nt, 4000 nt, and 2000 nt, respectively. The above mRNA was not purified.

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