T7 RNA Polymerase, GMP-Grade (GMP-T7P-EE101), DMF #037660

Catalog: GMP-T7P-EE101


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Product Description T7 RNA Polymerase (T7RNAP) is the key enzyme for in vitro transcription (IVT) of mRNA. T7 Polymerase is a DNA-dependent polymerase that efficiently incorporates standard or modified nucleoside triphosphates (NTPs) into RNA transcripts.
T7RNAP initiates transcription at the G* in the classic T7 promoter sequence-taatacgactcactataG*GG. T7 Polymerase is highly specific to its promoter sequence. When using the GAG cap1-Analog for co-transcription, taatacgactcactataA*GG is recommended.

Note: GMP T7 RNA Polymerase requires the cofactor Mg2+ for mRNA synthesis. We recommend restriction enzymes that produce blunt ends or 5'-overhangs.

We've filed our GMP T7RNAP with the FDA Drug Master Files (DMF) and its been assigned DMF #037660.
Synonyms T7RNAP; T7 Polymerase; T7 RNAP; GMP T7 Polymerase; GMP T7 RNA Polymerase; GMP T7RNAP
Application mRNA Synthesis
In vitro transcription (IVT)
RNA analysis
Radiolabeled RNA probe preparation
RNase protection assay
Concentration 50U/µL
Unit Definition One unit is defined as the amount of enzyme required to incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50µL in 1 hour at 37°C.
Source Expressed in an E.coli strain that carries the T7 RNA Polymerase gene.
Molecular Weight 98 kD
Quality Standards Activity (Molecular Beacon): ≥ 50 kU/mL
Purity (SEC-HPLC): ≥ 95%
Residual DNase: Negative
Residual RNase: Negative
Residual Protease: Negative
Endotoxin: ≤ 10 EU/mL
Residual Host Cell DNA: ≤ 100 pg/mg
Residual Host Protein: ≤ 20 ng/mg
Residual Heavy Metal: ≤ 10ppm
Residual Nickel Salt: ≤ 10 ppm
Bioburden: ≤ 1 CFU/10mL
Form Liquid
Shipping Shipped with dry ice
Stability And Storage -20±5°C for 24 months. Avoid repeated freeze-thaw cycles.
Figure 1. Three plasmids (lane 1 with poly A tail, lane 2 and 3 without poly A tail) were used as templates, and the reaction was performed for 2 hours at 37°C in a 20µL system. The transcription length was 2000 nt, 4000 nt, and 2000 nt, respectively. The above mRNA was not purified.
Figure 2. Detection of T7 RNA Polymerase by molecular beacon. When the molecular beacon and transcript specifically bind, the conformation changes and the fluorescence intensity changes. Three batches of T7 RNA Polymerase were detected, and the slope was similar across all batches, indicating stable activity across batches.
Figure 3. Yield was assessed via nanodrop and Agilent 4150, and purity was assessed by CE. Overall, yield and purity of KACTUS T7 was comparable to leading competitors.

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