T7 RNA Polymerase, GMP-Grade (GMP-T7P-EE101), DMF #037660
Catalog: GMP-T7P-EE101
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Product Description | T7 RNA Polymerase (T7RNAP) is the key enzyme for in vitro transcription (IVT) of mRNA. T7 Polymerase is a DNA-dependent polymerase that efficiently incorporates standard or modified nucleoside triphosphates (NTPs) into RNA transcripts.
T7RNAP initiates transcription at the G* in the classic T7 promoter sequence-taatacgactcactataG*GG. T7 Polymerase is highly specific to its promoter sequence. When using the GAG cap1-Analog for co-transcription, taatacgactcactataA*GG is recommended. Note: GMP T7 RNA Polymerase requires the cofactor Mg2+ for mRNA synthesis. We recommend restriction enzymes that produce blunt ends or 5'-overhangs. We've filed our GMP T7RNAP with the FDA Drug Master Files (DMF) and its been assigned DMF #037660. |
Synonyms | T7RNAP; T7 Polymerase; T7 RNAP; GMP T7 Polymerase; GMP T7 RNA Polymerase; GMP T7RNAP |
Application | mRNA Synthesis In vitro transcription (IVT) RNA analysis Radiolabeled RNA probe preparation Hybridization RNase protection assay |
Concentration | 50U/µL |
Unit Definition | One unit is defined as the amount of enzyme required to incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50µL in 1 hour at 37°C. |
Source | Expressed in an E.coli strain that carries the T7 RNA Polymerase gene. |
Molecular Weight | 98 kD |
Quality Standards | Activity (Molecular Beacon): ≥ 50 kU/mL
Purity (SEC-HPLC): ≥ 95% Residual DNase: Negative Residual RNase: Negative Residual Protease: Negative Endotoxin: ≤ 10 EU/mL Residual Host Cell DNA: ≤ 100 pg/mg Residual Host Protein: ≤ 20 ng/mg Residual Heavy Metal: ≤ 10ppm Residual Nickel Salt: ≤ 10 ppm Bioburden: ≤ 1 CFU/10mL |
Form | Liquid |
Shipping | Shipped with dry ice |
Stability And Storage | -20±5°C for 24 months. Avoid repeated freeze-thaw cycles. |
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