T7 RNA Polymerase, GMP grade (GMP-T7P-EE101)
|Product Description||T7 RNA Polymerase is the key enzyme for in vitro transcription of mRNA. It efficiently incorporates standard or modified nucleoside triphosphates (NTPs) into RNA transcripts. T7 RNA Polymerase initiates transcription at the G* in the classic T7 promoter-taatacgactcactataG*GG. When using GAG cap1-Analog for co-transcription, taatacgactcactataA*GG is recommended.
Restriction enzymes that produce blunt ends or 5'-overhangs are recommended.
The cofactor Mg2+ is required for the synthesis.
|Application||In vitro transcription, RNA analysis, Radiolabeled RNA probe preparation, Hybridization, RNase protection assay|
|Unit Definition||One unit is defined as the amount of enzyme required to incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50μl in 1 hour at 37°C.|
|Source||E.coli strain that carries the T7 RNA Polymerase gene|
|Quality Standards||Activity (Molecular Beacon): ≥ 50kU/mL
Purity (SEC-HPLC): ≥ 95%
Residual Endonuclease: Negative
Residual Exonuclease: Negative
Residual DNase: Negative
Residual RNase: Negative
Residual Protease: Negative
Endotoxin: ≤ 10EU/mL
Residual Host Cell DNA: ≤ 100pg/mL
Residual Host Protein: ≤ 20ng/mg
Residual Heavy Metal: ≤ 10ppm
Bioburden: ≤ 1CFU/10mL
|Shipping||Shipped with blue ice|
|Stability And Storage||-20±5°C for 24 months. Avoid repeated freeze-thaw cycles.|