SpCas9 D10A Nickase (CAS-EE111) Summary
Product Description |
Recombinant SpCas9 D10A Nickase, from Streptococcus pyogenes, a mutant version of Cas9, Cas9 nickase (Cas9n), generates single-strand nicks in DNA. Paired nickases, which effectively create DSBs by generating two single-strand breaks a short distance apart at a targeted site, have been introduced to reduce such off-target activity. |
Product Category |
Enzyme |
SpCas9 D10A Nickase (CAS-EE111) Specifications/Details
Applications |
Gene editing. |
Concentration |
10mg/ml |
Source |
E. coli with the Cas9 mutant (D10A) gene from Streptococcus pyogenes. |
Purity |
≥ 95% |
Endotoxin |
≤10 EU/mg |
Quality Standards |
Purity (Bis-Tris Page): ≥ 95% Purity (SEC-HPLC): ≥ 95% Purity (RP-HPLC): ≥ 95% Endotoxin: ≤10 EU/mg |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Stability And Storage |
Transport on dry ice. Store at -20±5°C. Avoid repeated freezing and thawing. |
Validation Images
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Figure 1. The in vitro cleavage activity data show that SpCas9 D10A Nickase can target and cleave more than 95% of the substrates. |
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Figure 3. The purity of Recombinant SpCas9 D10A Nickase detected by Bis-Tris PAGE is greater than 95%. |
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Figure 2. The purity of SpCas9 D10A Nickase detected by SEC-HPLC is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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