SpCas9 D10A Nickase (CAS-EE111)

Name: SpCas9 D10A Nickase (CAS-EE111)
Brand: KACTUS
SKU: CAS-EE111-100UG
Price: 150.0 USD
Availability: InStock

Catalog: CAS-EE111

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Product Description	Recombinant SpCas9 D10A Nickase, from Streptococcus pyogenes, a mutant version of Cas9, Cas9 nickase (Cas9n), generates single-strand nicks in DNA. Paired nickases, which effectively create DSBs by generating two single-strand breaks a short distance apart at a targeted site, have been introduced to reduce such off-target activity.
Product Category	Enzyme

Application	Gene editing.
Concentration	10mg/ml
Source	E. coli with the Cas9 mutant (D10A) gene from Streptococcus pyogenes.
Purity	≥ 95%
Endotoxin	≤10 EU/mg
Quality Standards	Purity (Bis-Tris Page): ≥ 95% Purity (SEC-HPLC): ≥ 95% Purity (RP-HPLC): ≥ 95% Endotoxin: ≤10 EU/mg

Form	Liquid
Stability And Storage	Transport on dry ice. Store at -20±5°C. Avoid repeated freezing and thawing.

	Figure 1. The in vitro cleavage activity data show that SpCas9 D10A Nickase can target and cleave more than 95% of the substrates.
	Figure 3. The purity of Recombinant SpCas9 D10A Nickase detected by Bis-Tris PAGE is greater than 95%.
	Figure 2. The purity of SpCas9 D10A Nickase detected by SEC-HPLC is greater than 95%.