MaxPure™ T7 RNA Polymerase (T7P-EE1MP)
  • Description: MaxPure™ T7 RNA Polymerase is an engineered T7 RNA polymerase. By mutating critical amino acids in the wild type sequence, MaxPure™ T7 RNA polymerase can efficiently reduce the generation of dsRNA byproducts. MaxPure™ T7 RNA polymerase uses single or double-strand DNAs harboring T7 promoter as templates to synthesize complementary RNAs in the presence of NTPs.
  • Applications: mRNA Synthesis
    In vitro transcription (IVT)
    RNA analysis
    Radiolabeled RNA probe preparation
    Hybridization
    RNase protection assay
  • Express System: E.coli
Catalog #T7P-EE1MP
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Product Description
 MaxPure™ T7 RNA Polymerase is an engineered T7 RNA polymerase. By mutating critical amino acids in the wild type sequence, MaxPure™ T7 RNA polymerase can efficiently reduce the generation of dsRNA byproducts. MaxPure™ T7 RNA polymerase uses single or double-strand DNAs harboring T7 promoter as templates to synthesize complementary RNAs in the presence of NTPs.

Note: MaxPure™ T7 RNA Polymerase is the commercial name for Premium T7 RNA Polymerase.
Applications
 mRNA Synthesis
In vitro transcription (IVT)
RNA analysis
Radiolabeled RNA probe preparation
Hybridization
RNase protection assay
Concentration
 200U/ul
Unit Definition
 One unit is defined as the amount of enzyme required to incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50µL in 1 hour at 37°C.
Source
 Expressed in an E.coli strain that carries the T7 RNA Polymerase gene.
Express System
 E.coli
Shipping
 Shipped with dry ice
Formulation
 liquid
Stability And Storage
 -20±5°C for 24 months. Avoid repeated freeze-thaw cycles.
Fig. 1 MaxPure™ T7 RNA Polymerase can reduce dsRNA production.
Fig. 2 Integrity assay.
Fig. 3 mRNA Yield assay.

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