supra-ABE (Adenine Base Editor) Summary
Product Description |
supra-ABE is an adenine base editor comprising the exclusively patented adenine deaminase eMa-TadA fused with Cas9 nickase (Cas9n), where eMa-TadA is embedded within Cas9n. The eMa-TadA deaminase catalyzes the deamination of adenine in DNA, converting it to inosine. During DNA replication, inosine is recognized as guanine, enabling A-to-G base substitution within editing window positions 4-14 without requiring a donor template or inducing DNA double-strand breaks. The supra-ABE system demonstrates high editing activity, broad effective editing windows, and low off-target rates, making it a versatile tool for applications in cell and gene therapy. |
Product Background |
Applications 1. Gene Knockout: Gene knockout is achieved by mutating the start codon ATG or altering the canonical splice sites GT-AG through A-to-G or T-to-C substitutions, thereby disrupting gene function. 2. Gene Correction: Precise repair of pathogenic single-base mutations (A-to-G or T-to-C substitutions at specific adenine or thymine sites) enables restoration of normal gene function through single-base editing. |
supra-ABE (Adenine Base Editor) Specifications/Details
Concentration |
10 mg/mL |
Source |
E. coli |
Molecular Weight |
183.83 kDa |
Purity |
≥ 80% |
Endotoxin |
≤ 10.0 EU/mg |
Quality Standards |
Concentration: 9.0-11.0mg/mL Purity (Bis-Tris PAGE): ≥ 80.0% Purity (SEC-HPLC): ≥ 80.0% Endotoxin: ≤ 10.0EU/mg |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Formulation |
30mM Tris, 300mM NaCl, 50% Glycerol, 0.1mM EDTA, 5mM DTT, pH 7.8 |
Stability And Storage |
Transport on dry ice. Store at -80 ±10°C. Avoid repeated freezing and thawing. |
Validation Images
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Sanger sequencing and EditR analysis demonstrate that supra-ABE efficiently mediates A-to-G conversion in the start codon (ATG) of PD1 and CD3G genes, disrupting translation initiation and achieving approximately 90% knockout efficiency. |
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Sanger sequencing and EditR analysis results show that supra-ABE can convert A to G within the editing window, with varying editing efficiencies for adenine (A) bases at different positions—the highest editing efficiency exceeds 90%. Additionally, the editing window of supra-ABE is wider than that of ABE8e. |
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FACS analysis demonstrated that supra-ABE efficiently knocked out the CD3G protein, achieving a knockout efficiency of 90.21%, whereas ABE8e exhibited no editing activity within the editing range at this site. |
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SEC-HPLC analysis confirms supra-ABE purity exceeding 95.0%. |
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Bis-Tris PAGE analysis confirms supra-ABE purity exceeding 95.0%. |
Other Available Proteins:
Finding available proteins...
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