HPLC chromatogram showing a sharp, predominant peak at approximately 10.24 minutes retention time, indicating over 95% purity of the recombinant Mouse GM-CSF protein expressed in E.coli without a tag.
Mouse GM-CSF Protein (GSF-ME001)
Mouse GM-CSF Protein (GSF-ME001)
Mouse GM-CSF Protein (GSF-ME001)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 6.32 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala18-Lys141
Catalog #GSF-ME001
Regular price $412.50
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Mouse GM-CSF Protein (GSF-ME001) Summary

Product Description

Recombinant Mouse GM-CSF Protein is expressed from E.coli without tag.It contains Ala18-Lys141.

Product Background

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim.

Product Category

Recombinant Protein / Cell therapy / Cell culture / Cytokines

Mouse GM-CSF Protein (GSF-ME001) Specifications/Details

Protein
GM-CSF
Synonyms
CSF; CSF2; GMCSF; GM-CSF; MGC131935; MGC138897
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Ala18-Lys141
Molecular Weight
The protein has a predicted MW of 14.11 kDa same as Bis-Tris PAGE result.
Activity
The affinity constant of 6.32 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

No Tag
Expression System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Mouse GM-CSF R alpha, His Tag captured on CM5 Chip via Anti-his antibody can bind Mouse GM-CSF, No Tag with an affinity constant of 6.32 nM as determined in SPR assay (Biacore T200). Mouse GM-CSF R alpha, His Tag captured on CM5 Chip via Anti-his antibody can bind Mouse GM-CSF, No Tag with an affinity constant of 6.32 nM as determined in SPR assay (Biacore T200).
Mouse GM-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse GM-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse GM-CSF is greater than 95% as determined by SEC-HPLC. The purity of Mouse GM-CSF is greater than 95% as determined by SEC-HPLC.
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