SapI V2.0 (SAP-SE102) Summary
Product Description |
SapI V2.0 is an IIS-type restriction endonuclease that can recognize non-palindromic DNA motifs and create sticky ends after cutting the DNA domain outside the recognition motif, similar to BspQI, PciSI, and LguI. Compared to SapI(Catalog No. SAP-SE101), the SapI V2 has been engineered to digest ‘difficult to cut’ plasmids with higher cutting efficiency. The 10×Cut Reaction Buffer contains recombinant Albumin (rAlbumin), ensuring the stability and safety of the product. |
SapI V2.0 (SAP-SE102) Specifications/Details
Synonyms |
Sap1 |
Applications |
Restriction site mapping
Genotyping SNP |
Concentration |
10U/µL |
Unit Definition |
One unit (1U) is defined as the amount of enzyme required to digest 1 μg of λ DNA in 4 hour at 37℃ in a total reaction volume of 50 μl. |
Source |
E. coli |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with blue ice |
Stability And Storage |
-20±5°C for 24 months. Avoid repeated freeze-thaw cycles. |
Validation Images
|
|
SapI restriction enzyme recognition and cleavage site showing asymmetric cut pattern with 5' GCTCTTC(N)1↓ and complementary 3' CGAGAAG(N)4↑ overhang |
Other Available Proteins:
Finding available proteins...
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