• Description: SapI V2.0 is an IIS-type restriction endonuclease that can recognize non-palindromic DNA motifs and create sticky ends after cutting the DNA domain outside the recognition motif, similar to BspQI, PciSI, and LguI. Compared to SapI(Catalog No. SAP-SE101), the SapI V2 has been engineered to digest ‘difficult to cut’ plasmids with higher cutting efficiency. The 10×Cut Reaction Buffer contains recombinant Albumin (rAlbumin), ensuring the stability and safety of the product.
  • Applications: Restriction site mapping
    Genotyping
    SNP
Catalog #SAP-SE102
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Product Description
 SapI V2.0 is an IIS-type restriction endonuclease that can recognize non-palindromic DNA motifs and create sticky ends after cutting the DNA domain outside the recognition motif, similar to BspQI, PciSI, and LguI. Compared to SapI(Catalog No. SAP-SE101), the SapI V2 has been engineered to digest ‘difficult to cut’ plasmids with higher cutting efficiency. The 10×Cut Reaction Buffer contains recombinant Albumin (rAlbumin), ensuring the stability and safety of the product.
Synonyms
 Sap1
Applications
 Restriction site mapping
Genotyping
SNP
Concentration
 10U/µL
Unit Definition
 One unit (1U) is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37℃ in a total reaction volume of 50 μl.
Source
 E. coli
Biotinylated
 no
Form
 Liuid
Shipping
 Shipped with blue ice
Stability And Storage
 -20±5°C for 24 months. Avoid repeated freeze-thaw cycles.
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