SapI V2.0 (SAP-SE102)

  • Description: SapI V2.0 is an IIS-type restriction endonuclease that can recognize non-palindromic DNA motifs and create sticky ends after cutting the DNA domain outside the recognition motif, similar to BspQI, PciSI, and LguI. Compared to SapI(Catalog No. SAP-SE101), the SapI V2 has been engineered to digest ‘difficult to cut’ plasmids with higher cutting efficiency. The 10×Cut Reaction Buffer contains recombinant Albumin (rAlbumin), ensuring the stability and safety of the product.
  • Applications: Restriction site mapping
    Genotyping
    SNP

Catalog: SAP-SE102

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Product Description SapI V2.0 is an IIS-type restriction endonuclease that can recognize non-palindromic DNA motifs and create sticky ends after cutting the DNA domain outside the recognition motif, similar to BspQI, PciSI, and LguI. Compared to SapI(Catalog No. SAP-SE101), the SapI V2 has been engineered to digest ‘difficult to cut’ plasmids with higher cutting efficiency. The 10×Cut Reaction Buffer contains recombinant Albumin (rAlbumin), ensuring the stability and safety of the product.
Synonyms Sap1
Application Restriction site mapping
Genotyping
SNP
Concentration 10U/µL
Unit Definition One unit (1U) is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37℃ in a total reaction volume of 50 μl.
Source E. coli
Form Liuid
Shipping Shipped with blue ice
Stability And Storage -20±5°C for 24 months. Avoid repeated freeze-thaw cycles.

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