Name: Biotinylated Human FGF1 Protein (FGF-HE40AB)
Brand: KactusBio
SKU: FGF-HE40AB-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human FGF1 Protein (FGF-HE40AB)

Immobilized Human FGFR1 alpha (IIIc), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGF1, His-Avi Tag with the EC50 of 100.7ng/ml determined by ELISA (QC Test).

Biotinylated Human FGF1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: E.coli
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human FGFR1 alpha (IIIc), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGF1, His-Avi Tag with the EC50 of 100.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Phe16-Asp155

Catalog #FGF-HE40AB

Size

100UG

500UG

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Biotinylated Human FGF1 Protein (FGF-HE40AB) Summary

Product Description	Recombinant Biotinylated Human FGF1 Protein is expressed from E.coli with His tag and Avi tag at the N-terminus.It contains Phe16-Asp155.
Product Background	Fibroblast Growth Factor 1 (Fgf1), also known as acidic FGF (aFGF), is involved in the regulation of various biological processes, ranging from development to disease pathogenesis. It is a single chain polypeptide and is highly expressed in adult brain and kidney tissues. Its expression has been shown to be directed by multiple tissue-specific promoters, which generate transcripts of varying lengths.
Product Category	Recombinant Protein / Cell therapy / Cell culture / Cytokines

Biotinylated Human FGF1 Protein (FGF-HE40AB) Specifications/Details

Protein	FGF1
Synonyms	FGF-1; aFGF; ECGF; HBGF-1; FGFA
Accession	P05230-1
Species	Human
Biotinylated	yes
Amino Acid Range	Phe16-Asp155
Molecular Weight	The protein has a predicted MW of 18.74 kDa. Due to glycosylation, the protein migrates to 20-24 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human FGFR1 alpha (IIIc), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGF1, His-Avi Tag with the EC50 of 100.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-His-Avi
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human FGFR1 alpha (IIIc), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGF1, His-Avi Tag with the EC50 of 100.7ng/ml determined by ELISA (QC Test).
	Biotinylated Human FGF1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.