Product Description |
Recombinant Biotinylated Human FGF1 Protein is expressed from E.coli with His tag and Avi tag at the N-terminus.It contains Phe16-Asp155. |
Product Background |
Fibroblast Growth Factor 1 (Fgf1), also known as acidic FGF (aFGF), is involved in the regulation of various biological processes, ranging from development to disease pathogenesis. It is a single chain polypeptide and is highly expressed in adult brain and kidney tissues. Its expression has been shown to be directed by multiple tissue-specific promoters, which generate transcripts of varying lengths. |
Product Category |
Recombinant Protein / Cell therapy / Cell culture |
Protein |
FGF1 |
Synonyms |
FGF-1; aFGF; ECGF; HBGF-1; FGFA |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Phe16-Asp155 |
Molecular Weight |
The protein has a predicted MW of 18.74 kDa. Due to glycosylation, the protein migrates to 20-24 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human FGFR1 alpha (IIIc), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGF1, His-Avi Tag with the EC50 of 60.0ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-His-Avi |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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|
Immobilized Human FGFR1 alpha (IIIc), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGF1, His-Avi Tag with the EC50 of 60.0ng/ml determined by ELISA (QC Test). |
|
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Biotinylated Human FGF1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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