Human/Cynomolgus Activin RIIB/ACVR2B Protein (ARB-HM12B)
Human/Cynomolgus Activin RIIB/ACVR2B Protein (ARB-HM12B)
Human/Cynomolgus Activin RIIB/ACVR2B Protein (ARB-HM12B)
Human/Cynomolgus Activin RIIB/ACVR2B Protein (ARB-HM12B)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human Activin A, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 21.4 ng/ml determined by ELISA (QC Test). The affinity constant of 27.92 pM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ser19-Thr137 (Human)/Ser40-Thr158 (Cynomolgus)
Catalog #ARB-HM12B
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Product Description
 Recombinant Human/Cynomolgus Activin RIIB/ACVR2B Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus).
Product Background
 ActRIIB (activin receptor type-2B) is an activin receptor subtype constitutively expressed in the whole body, playing a role in cellular proliferation, differentiation, and metabolism. For its various physiological activities, ActRIIB interacts with activin and multiple other ligands including myostatin (MSTN), growth differentiation factor 11 (GDF11), and bone morphogenetic protein 9 (BMP9).
Product Category
 Recombinant Protein
Target
 Activin RIIB/ACVR2B
Synonyms
 Activin receptor type-2B; ACVR2B; Activin RIIB
Accession
Species
 Human/Cynomolgus
Biotinylated
 no
Amino Acid Range
 Ser19-Thr137 (Human)/Ser40-Thr158 (Cynomolgus)
Molecular Weight
 The protein has a predicted MW of 14.75 kDa. Due to glycosylation, the protein migrates to 25-45 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Activin A, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 21.4 ng/ml determined by ELISA (QC Test). The affinity constant of 27.92 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human/Cynomolgus Activin RIIB, His Tag captured on CM5 Chip via anti-his antibody can bind Human Activin A- with an affinity constant of 27.92 pM as determined in SPR assay (Biacore T200).
Immobilized Human Activin A, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 21.4 ng/ml determined by ELISA (QC Test).
Immobilized Human/Cynomolgus Activin RIIB, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-Activin RIIB Antibody, hFc Tag with the EC50 of 4.3 ng/ml determined by ELISA.
Human/Cynomolgus Activin RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human/Cynomolgus Activin RIIB is greater than 95% as determined by SEC-HPLC.

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