Name: Human/Cynomolgus Activin RIIB/ACVR2B Protein (ARB-HM12B)
Brand: KactusBio
SKU: ARB-HM12B-100UG
Price: 165.0 USD
Availability: InStock

Human/Cynomolgus Activin RIIB/ACVR2B Protein (ARB-HM12B)

Human/Cynomolgus Activin RIIB, His Tag captured on CM5 Chip via anti-his antibody can bind Human Activin A- with an affinity constant of 27.92 pM as determined in SPR assay (Biacore T200).

Immobilized Human Activin A, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 15.3 ng/ml determined by ELISA (QC Test).

Immobilized Human/Cynomolgus Activin RIIB, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Bimagrumab with the EC50 of 4.3 ng/ml determined by ELISA.

Human/Cynomolgus Activin RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Serial dilutions of Bimagrumab were added into Human Activin RIIB, His Tag : Biotinylated Human Activin A, Avi Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.14ug/ml.

The purity of Human/Cynomolgus Activin RIIB is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Activin A, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 15.3 ng/ml determined by ELISA (QC Test). The affinity constant of 27.92 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus)

Catalog #ARB-HM12B

Size

100UG

500UG

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Product Description	Recombinant Human/Cynomolgus Activin RIIB/ACVR2B Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus).
Product Background	ActRIIB (activin receptor type-2B) is an activin receptor subtype constitutively expressed in the whole body, playing a role in cellular proliferation, differentiation, and metabolism. For its various physiological activities, ActRIIB interacts with activin and multiple other ligands including myostatin (MSTN), growth differentiation factor 11 (GDF11), and bone morphogenetic protein 9 (BMP9).
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	Activin RIIB/ACVR2B
Synonyms	Activin receptor type-2B; ACVR2B; Activin RIIB; ActRIIB
Accession	Q13705-1(Human) / XP_045242398.1(Cynomolgus)
Species	Human/Cynomolgus
Biotinylated	no
Amino Acid Range	Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus)
Molecular Weight	The protein has a predicted MW of 14.75 kDa. Due to glycosylation, the protein migrates to 25-45 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Activin A, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 15.3 ng/ml determined by ELISA (QC Test). The affinity constant of 27.92 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human/Cynomolgus Activin RIIB, His Tag captured on CM5 Chip via anti-his antibody can bind Human Activin A- with an affinity constant of 27.92 pM as determined in SPR assay (Biacore T200).
	Immobilized Human Activin A, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 15.3 ng/ml determined by ELISA (QC Test).
	Immobilized Human/Cynomolgus Activin RIIB, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Bimagrumab with the EC50 of 4.3 ng/ml determined by ELISA.
	Human/Cynomolgus Activin RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Serial dilutions of Bimagrumab were added into Human Activin RIIB, His Tag : Biotinylated Human Activin A, Avi Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.14ug/ml.
	The purity of Human/Cynomolgus Activin RIIB is greater than 95% as determined by SEC-HPLC.

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