Human/Cynomolgus Activin RIIB/ACVR2B Protein, Ultra Low Endotoxin (ARB-HM12B-UL)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag (Cat. ACV-HM401B) with the EC50 of 3.2ng/ml determined by ELISA. The affinity constant of 27.92 pM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus)
Catalog #ARB-HM12B-UL
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Size
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Human/Cynomolgus Activin RIIB/ACVR2B Protein, Ultra Low Endotoxin (ARB-HM12B-UL) Summary

Product Description

 Recombinant Human/Cynomolgus Activin RIIB/ACVR2B Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus).

Product Background

 ActRIIB (activin receptor type-2B) is an activin receptor subtype constitutively expressed in the whole body, playing a role in cellular proliferation, differentiation, and metabolism. For its various physiological activities, ActRIIB interacts with activin and multiple other ligands including myostatin (MSTN), growth differentiation factor 11 (GDF11), and bone morphogenetic protein 9 (BMP9).

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human/Cynomolgus Activin RIIB/ACVR2B Protein, Ultra Low Endotoxin (ARB-HM12B-UL) Specifications/Details

Protein
 Activin RIIB/ACVR2B
Synonyms
 Activin receptor type-2B; ACVR2B; Activin RIIB; ActRIIB
Accession
Species
 Human/Cynomolgus
Biotinylated
 no
Amino Acid Range
 Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus)
Molecular Weight
 The protein has a predicted MW of 14.75 kDa. Due to glycosylation, the protein migrates to 25-45 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag (Cat. ACV-HM401B) with the EC50 of 3.2ng/ml determined by ELISA. The affinity constant of 27.92 pM as determined in SPR assay (Biacore T200).
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human/Cynomolgus Activin RIIB, His Tag captured on CM5 Chip via anti-his antibody can bind Human Activin A with an affinity constant of 27.92 pM as determined in SPR assay (Biacore T200). Human/Cynomolgus Activin RIIB, His Tag captured on CM5 Chip via anti-his antibody can bind Human Activin A with an affinity constant of 27.92 pM as determined in SPR assay (Biacore T200).
Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag (Cat. ACV-HM401B) with the EC50 of 3.2ng/ml determined by ELISA. Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag (Cat. ACV-HM401B) with the EC50 of 3.2ng/ml determined by ELISA.
Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GDF-8, Avi Tag with the EC50 of 2.7ng/ml determined by ELISA. Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GDF-8, Avi Tag with the EC50 of 2.7ng/ml determined by ELISA.
Immobilized Human/Cynomolgus Activin RIIB, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Bimagrumab with the EC50 of 4.3 ng/ml determined by ELISA. Immobilized Human/Cynomolgus Activin RIIB, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Bimagrumab with the EC50 of 4.3 ng/ml determined by ELISA.
Human/Cynomolgus Activin RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human/Cynomolgus Activin RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Serial dilutions of Bimagrumab were added into Human/Cynomolgus Activin RIIB, His Tag : Biotinylated Human Activin A, Avi Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.14ug/ml. Serial dilutions of Bimagrumab were added into Human/Cynomolgus Activin RIIB, His Tag : Biotinylated Human Activin A, Avi Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.14ug/ml.
The purity of Human/Cynomolgus Activin RIIB is greater than 95% as determined by SEC-HPLC. The purity of Human/Cynomolgus Activin RIIB is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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