• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FGFR3 alpha (IIIb), His Tag (Cat. FGF-HM43B) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human FGF4, hFc Tag with the EC50 of 4.29ug/ml determined by ELISA.
  • Amino Acid Range: Ala31-Leu206
Catalog #FGF-HM204
Price
Regular price $315.00
Regular price Sale price $315.00
/
Size
Avantor VWR Fisher Scientific

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Human FGF4 Protein (FGF-HM204) Summary

Product Description

 Recombinant Human FGF4 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Ala31-Leu206.

Product Background

 Fibroblast growth factor-4 (FGF-4), a highly mitogenic protein encoded by the k-fgf/hst oncogene, stimulates the growth of a variety of cells of mesenchymal and neuroectodermal origin. It is a member of the FGF family and plays several important roles in bone development during embryogenesis.

Product Category

 Recombinant Protein / Cell therapy / Cell culture / Cytokines

Human FGF4 Protein (FGF-HM204) Specifications/Details

Protein
 FGF4
Synonyms
 FGF-4; FGF 4; HST; HST-1; HSTF-1; HBGF-4; KS3
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala31-Leu206
Molecular Weight
 The protein has a predicted MW of 45.23 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human FGFR3 alpha (IIIb), His Tag (Cat. FGF-HM43B) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human FGF4, hFc Tag with the EC50 of 4.29ug/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human FGFR3 alpha (IIIb), His Tag (Cat. FGF-HM43B) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human FGF4, hFc Tag with the EC50 of 4.29ug/ml determined by ELISA. Immobilized Human FGFR3 alpha (IIIb), His Tag (Cat. FGF-HM43B) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human FGF4, hFc Tag with the EC50 of 4.29ug/ml determined by ELISA.
Human FGF4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human FGF4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human FGF4 is greater than 95% as determined by SEC-HPLC. The purity of Human FGF4 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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