Human FGF8a-Protein (FGF-HE08A)
Human FGF8a-Protein (FGF-HE08A)
Human FGF8a-Protein (FGF-HE08A)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Amino Acid Range: Gln23-Arg204
Catalog #FGF-HE08A
Regular price $300.00
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Size

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Product Description

Recombinant Human FGF8a-Protein is expressed from E.coli without tag.It contains Gln23-Arg204.

Product Background

Two of the four human FGF8 splice isoforms, FGF8a and FGF8b, are expressed in the mid-hindbrain region during development. Although the only difference between these isoforms is the presence of an additional 11 amino acids at the N terminus of FGF8b, these isoforms possess remarkably different abilities to pattern the midbrain and anterior hindbrain.

Product Category

Recombinant Protein / Cell therapy / Cell culture

Protein

FGF8a

Synonyms

FGF8a; FGF-8a; FGF-8; FGF8; FGF 8; AIGF; HBGF-8; HBGF8; HBGF 8;

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gln23-Arg204

Molecular Weight

The protein has a predicted MW of 21.20 kDa same as Bis-Tris PAGE result.

Product Tag

No Tag

Expression System

E.coli

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 0.01 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 20mM Tris-HCl, 300mM NaCl (pH 8.5). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human FGF8a-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human FGF8a-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblasts. The ED50-for this effect is 0.5 - 2 ug/mL. Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblasts. The ED50-for this effect is 0.5 - 2 ug/mL.

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