Product Description |
Recombinant Human Latent Activin A Protein is expressed from HEK293 with His tag at the N-terminus.It contains Ser21-Ser426. |
Product Background |
Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression. |
Product Category |
Recombinant Protein |
Target |
Latent Activin A |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser21-Ser426 |
Molecular Weight |
The protein has a predicted MW of 13.0 kDa (mature form) and 32.0 kDa (pro form). Due to glycosylation and interchain disulfide bond, the protein migrates to 15 kDa (mature form) and 40-50 kDa (pro form) under reduced (R) condition based on Bis-Tris PAGE result. |
Product Tag |
N-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human Latent Activin A on Bis-Tris PAGE under reduced (R) condition. The purity is greater than 95%. |
|
|
Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50-for this effect is 30-40 ng/mL. |
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