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Human Latent Activin A Protein (ACV-HM101) Summary
Product Description |
Recombinant Human Latent Activin A Protein is expressed from HEK293 with His tag at the N-terminus.It contains Ser21-Ser426. |
Product Background |
Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human Latent Activin A Protein (ACV-HM101) Specifications/Details
Protein |
Activin A |
Synonyms |
INHBA; Activin A; INHBA; Activin A; Inhibin beta A chain |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser21-Ser426 |
Molecular Weight |
The protein has a predicted MW of 13.0 kDa (mature form) and 32.0 kDa (pro form). Due to glycosylation and interchain disulfide bond, the protein migrates to 15 kDa (mature form) and 40-50 kDa (pro form) under reduced (R) condition based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Latent Activin A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 16.6ng/ml determined by ELISA. |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human Latent Activin A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 16.6ng/ml determined by ELISA. |
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Immobilized Human Latent Activin A, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag with the EC50 of 23.5ng/ml determined by ELISA. |
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Human Latent Activin A on Bis-Tris PAGE under reduced (R) condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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