Name: Human SCF Protein, Ultra Low Endotoxin (SCF-HE001-UL)
Brand: KactusBio
SKU: SCF-HE001-UL-100UG
Price: 245.0 USD
Availability: InStock

Human SCF Protein, Ultra Low Endotoxin (SCF-HE001-UL)

Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.55ug/ml determined by ELISA (QC Test).

Human SCF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Measured in a cell proliferation assay using Mo7e cells. The ED50-for this effect is 1-8 ng/mL (QC Test).

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.55ug/ml determined by ELISA (QC Test).
Amino Acid Range: Glu26-Ala189

Catalog #SCF-HE001-UL

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human SCF Protein, Ultra Low Endotoxin (SCF-HE001-UL) Summary

Product Description	Recombinant Human SCF Protein is expressed from E.coli without tag.It contains Glu26-Ala189.
Product Background	Kit-ligand (Kitl) also known as steel factor, stem cell factor and mast cell growth factor plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. Kitl exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes.
Product Category	Recombinant Protein / Cell therapy / Cell culture / Cytokines

Human SCF Protein, Ultra Low Endotoxin (SCF-HE001-UL) Specifications/Details

Protein	SCF
Synonyms	MGF; c-Kit ligand; sKITLG; KITLG; FPH2; KL-1; Kitl; SF; SHEP7; KL
Accession	P21583-1
Species	Human
Biotinylated	no
Amino Acid Range	Glu26-Ala189
Molecular Weight	The protein has a predicted MW of 18.45 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.55ug/ml determined by ELISA (QC Test).
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.55ug/ml determined by ELISA (QC Test).
	Human SCF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured in a cell proliferation assay using Mo7e cells. The ED50-for this effect is 1-8 ng/mL (QC Test).