- Express System: HEK293
- Product Tag: C-His
- Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
- Activity: Immobilized Human Activin A, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Rat Activin RIIA, His Tag with the EC50 of 0.36ung/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: Ala20-Pro135
Product Description |
Recombinant Rat Activin RIIA/ACVR2A Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala20-Pro135. |
Product Background |
Activin A receptor type 2A (ACVR2A) is a membrane receptor in the transforming growth factor- beta (TGF-β signaling pathway, which is involved in the regulation of cell proliferation, migration, and apoptosis. Loss of ACVR2A has an important role in cancer progression and distant metastasis and may serve as a prognostic marker in patients with colon cancer. |
Product Category |
Recombinant Protein |
Target |
Activin RIIA/ACVR2A |
Synonyms |
ACTR-IIA; ACTRIIA; ACVR2A; ACVR2 |
Accession |
|
Species |
Rat |
Biotinylated |
no |
Amino Acid Range |
Ala20-Pro135 |
Molecular Weight |
The protein has a predicted MW of 15.06 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Activin A, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Rat Activin RIIA, His Tag with the EC50 of 0.36ung/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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