Murine RNase Inhibitor, GMP grade (GMP-RNI-ME101)
Catalog: GMP-RNI-ME101
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| Product Description | RNase inhibitors are essential to protect RNA from RNase contamination. It inhibits the enzymatic activity of RNases by noncovalently binding to the active site. Murine RNase inhibitor is a recombinant RNase inhibitor of murine origin expressed and purified in E.coli. It specifically inactivates RNases such as RNase A, RNase B, and RNase C by binding covalently with RNase to form a 1:1 complex. Murine RNase Inhibitor is compatible with RNA synthesis, RT-PCR and other RNA-related reactions. |
| Application | In Vitro Transcription RT-PCR cDNA synthesis |
| Concentration | 40U/µL |
| Unit Definition | One unit is defined as the amount of Murine RNase Inhibitor required to inhibit the activity of 5ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A. |
| Source | Expressed in an E.coli strain with the RNase inhibitor gene from mouse. |
| Molecular Weight | 50 kD |
| Quality Standards | Activity (Ultraviolet spectrophotometry): ≥ 40 kU/mL
Purity (SEC-HPLC): ≥ 95% Residual Endonuclease: Negative Residual Exonuclease: Negative Residual RNase: Negative Residual Protease: Negative Endotoxin: ≤ 10 EU/mL Residual Host Cell DNA: ≤ 100 pg/mg Residual Host Protein: ≤ 50 ng/mg Bioburden: ≤ 1 CFU/10mL |
| Form | Liquid |
| Shipping | Shipped with blue ice |
| Stability And Storage | -20±5°C for 12 months. Avoid repeated freeze-thaw cycles. |
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