Murine RNase Inhibitor, GMP grade (GMP-RNI-ME101)

Catalog: GMP-RNI-ME101


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Product Description RNase inhibitors are essential to protect RNA from RNase contamination. It inhibits the enzymatic activity of RNases by noncovalently binding to the active site. Murine RNase inhibitor is a recombinant RNase inhibitor of murine origin expressed and purified in E.coli. It specifically inactivates RNases such as RNase A, RNase B, and RNase C by binding covalently with RNase to form a 1:1 complex. Murine RNase Inhibitor is compatible with RNA synthesis, RT-PCR and other RNA-related reactions.
Application In Vitro Transcription
cDNA synthesis
Concentration 40U/µL
Unit Definition One unit is defined as the amount of Murine RNase Inhibitor required to inhibit the activity of 5ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A.
Source Expressed in an E.coli strain with the RNase inhibitor gene from mouse.
Molecular Weight 50 kD
Quality Standards Activity (Ultraviolet spectrophotometry): ≥ 40 kU/mL
Purity (SEC-HPLC): ≥ 95%
Residual Endonuclease: Negative
Residual Exonuclease: Negative
Residual RNase: Negative
Residual Protease: Negative
Endotoxin: ≤ 10 EU/mL
Residual Host Cell DNA: ≤ 100 pg/mg
Residual Host Protein: ≤ 50 ng/mg
Bioburden: ≤ 1 CFU/10mL
Form Liquid
Shipping Shipped with blue ice
Stability And Storage -20±5°C for 12 months. Avoid repeated freeze-thaw cycles.

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