Murine RNase Inhibitor, GMP-Grade (GMP-RNI-ME101), DMF #038031
  • Description: Inhibits the enzymatic activity of RNases by noncovalently binding to the active site
  • Applications: In Vitro Transcription
    RT-PCR
    cDNA synthesis
  • Quality: FDA Drug Master Files #038031
    Manufactured according to GMP guidelines
    Antibiotic-free and animal-free production
    Suitable for manufacturing of mRNA therapeutics and vaccines
Catalog #GMP-RNI-ME101
Regular price $1,300.00
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Product Description

RNase inhibitors are essential to protect RNA from RNase contamination. It inhibits the enzymatic activity of RNases by noncovalently binding to the active site. Murine RNase inhibitor is a recombinant RNase inhibitor of murine origin expressed and purified in E.coli. It specifically inactivates RNases such as RNase A, RNase B, and RNase C by binding covalently with RNase to form a 1:1 complex. Murine RNase Inhibitor is compatible with RNA synthesis, RT-PCR and other RNA-related reactions.

This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF #038031.

Applications

In Vitro Transcription
RT-PCR
cDNA synthesis

Concentration

120U/µL

Unit Definition

One unit is defined as the amount of Murine RNase Inhibitor required to inhibit the activity of 5ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A.

Source

Expressed in an E.coli strain with the RNase inhibitor gene from mouse.

Biotinylated

no

Molecular Weight

62.1 kD

Quality Standards

Activity (Ultraviolet spectrophotometry): ≥ 120 kU/mL
Purity (SEC-HPLC): ≥ 95%
Residual Endonuclease: Negative
Residual Exonuclease: Negative
Residual RNase: Negative
Residual Protease: Negative
Endotoxin: ≤ 10 EU/mL
Residual Host Cell DNA: ≤ 100 pg/mg
Residual Host Protein: ≤ 50 ng/mg
Bioburden: ≤ 1 CFU/10mL
Form
Liquid
Shipping
Shipped with dry ice
Stability And Storage
-20±5°C for 24 months. Avoid repeated freeze-thaw cycles.
Murine RNase Inhibitor Structure. Murine RNase Inhibitor Structure.

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