Product Description |
RNase inhibitors are essential to protect RNA from RNase contamination. It inhibits the enzymatic activity of RNases by noncovalently binding to the active site. Murine RNase inhibitor is a recombinant RNase inhibitor of murine origin expressed and purified in E.coli. It specifically inactivates RNases such as RNase A, RNase B, and RNase C by binding covalently with RNase to form a 1:1 complex. Murine RNase Inhibitor is compatible with RNA synthesis, RT-PCR and other RNA-related reactions. This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF #038031. |
Applications |
In Vitro Transcription RT-PCR cDNA synthesis |
Concentration |
120U/µL |
Unit Definition |
One unit is defined as the amount of Murine RNase Inhibitor required to inhibit the activity of 5ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A. |
Source |
Expressed in an E.coli strain with the RNase inhibitor gene from mouse. |
Biotinylated |
no |
Molecular Weight |
62.1 kD |
Quality Standards |
Activity (Ultraviolet spectrophotometry): ≥ 120 kU/mL
Purity (SEC-HPLC): ≥ 95% Residual Endonuclease: Negative Residual Exonuclease: Negative Residual RNase: Negative Residual Protease: Negative Endotoxin: ≤ 10 EU/mL Residual Host Cell DNA: ≤ 100 pg/mg Residual Host Protein: ≤ 50 ng/mg Bioburden: ≤ 1 CFU/10mL |
Form |
Liquid |
Shipping |
Shipped with dry ice |
Stability And Storage |
-20±5°C for 24 months. Avoid repeated freeze-thaw cycles. |
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Murine RNase Inhibitor Structure. |
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