Product Description |
Recombinant Human Activin A Protein is expressed from HEK293 without tag.It contains Gly311-Ser426. |
Product Background |
Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression. |
Product Category |
Recombinant Protein |
Target |
Activin A |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly311-Ser426 |
Molecular Weight |
The protein has a predicted MW of 12.97 kDa. Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 23-25 kDa under Non reducing (N) condition based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Activin RIIB, His Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test). The affinity constant of 55.35 pM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
No Tag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 4mM HCI. Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCI. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated Human Activin RIIB, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human Activin A, No Tag with an affinity constant of 55.35 pM as determined in SPR assay (Biacore T200). |
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Immobilized Human Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Activin RIIB, His Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test). |
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Immobilized Human Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA. |
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Human Activin A on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%. |
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|
Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL (QC Test). |
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