Human/Mouse/Rat Activin A Protein (ACV-HM001)
Human/Mouse/Rat Activin A Protein (ACV-HM001)
Human/Mouse/Rat Activin A Protein (ACV-HM001)
Human/Mouse/Rat Activin A Protein (ACV-HM001)
Human/Mouse/Rat Activin A Protein (ACV-HM001)
Human/Mouse/Rat Activin A Protein (ACV-HM001)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human/Mouse/Rat Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test). The affinity constant of 55.35 pM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly311-Ser426
Catalog #ACV-HM001
Regular price $247.50
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Product Description

Recombinant Human/Mouse/Rat Activin A Protein is expressed from HEK293 without tag.It contains Gly311-Ser426.

Product Background

Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
Activin A
Synonyms
INHBA; Activin A; Inhibin beta A chain;
Accession
Species
Human/Mouse/Rat
Biotinylated
no
Amino Acid Range
Gly311-Ser426
Molecular Weight
The protein has a predicted MW of 12.97 kDa. Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 23-30 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Activity
Immobilized Human/Mouse/Rat Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test). The affinity constant of 55.35 pM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

No Tag
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 0.1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 4mM HCI. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in 4mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human/Cynomolgus Activin RIIB, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human/Mouse/Rat Activin A, No Tag with an affinity constant of 55.35 pM as determined in SPR assay (Biacore T200). Biotinylated Human/Cynomolgus Activin RIIB, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human/Mouse/Rat Activin A, No Tag with an affinity constant of 55.35 pM as determined in SPR assay (Biacore T200).
Human ACVR1B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human Activin A, No Tag with an affinity constant of 18.43 nM as determined in SPR assay (Biacore T200). Human ACVR1B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human Activin A, No Tag with an affinity constant of 18.43 nM as determined in SPR assay (Biacore T200).
Immobilized Human/Mouse/Rat Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test). Immobilized Human/Mouse/Rat Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test).
Immobilized Human/Mouse/Rat Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA. Immobilized Human/Mouse/Rat Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA.
Human/Mouse/Rat Activin A on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%. Human/Mouse/Rat Activin A on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL (QC Test). Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL (QC Test).
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