Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM105)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD3E&CD3D, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.36nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)
Catalog #CD3-HM105
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Product Description
 Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp23-Asp126 (CD3E) & Phe22-Ala105 (CD3D).
Product Background
 T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Product Category
 Recombinant Protein
Target
 CD3E&CD3D/CD3 epsilon&CD3 delta
Synonyms
 CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)
Molecular Weight
 The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 28-38 kDa and 25 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD3E&CD3D, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.36nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CD3E&CD3D, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.36nM as determined in SPR assay (Biacore T200).
Immobilized Human CD3E&CD3D, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test).
Immobilized Human CD3E&CD3D, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
Human CD3E&CD3D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.

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