Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM105)
Catalog: CD3-HM105
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| Product Description | Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp23-Glu120 (CD3E)&Phe22-Ala105 (CD3D). |
Product Background |
T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. |
Product Category |
Recombinant Protein |
Target |
CD3E&CD3D/CD3 epsilon&CD3 delta |
| Synonyms | CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon |
Gene/Protein ID |
P07766(CD3E)&P04234(CD3D) |
Species |
Human |
Exact Sequence |
Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D) |
| Molecular Weight | The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 28-38 kDa and 25 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human CD3E&CD3D at 2ug/ml (100ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.36nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human CD3E&CD3D, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.36nM as determined in SPR assay (Biacore T200). |
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Immobilized Human CD3E&CD3D at 2ug/ml (100ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test). |
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Immobilized Human CD3E&CD3D at 2ug/ml (100ul/Well). Dose response curve for Foralumab, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA. |
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The purity of Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC. |
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Human CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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