Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM105)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD3E&CD3D at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 38ng/ml determined by ELISA. The affinity constant of 0.36nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D)

Catalog: CD3-HM105

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Product Description Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from Expi293 with His tag at the C-terminal.It contains Asp23-Glu120 (CD3E)&Phe22-Ala105 (CD3D).
Product Category Recombinant Protein
Target CD3E&CD3D/CD3 epsilon&CD3 delta
Synonyms CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon
Gene/Protein ID P07766(CD3E)&P04234(CD3D)
Species Human
Exact Sequence Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D)
Molecular Weight The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CD3E&CD3D at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 38ng/ml determined by ELISA. The affinity constant of 0.36nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CD3E&CD3D, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.36nM as determined in SPR assay (Biacore T200).
Immobilized Human CD3E&CD3D at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 38ng/ml determined by ELISA.
Immobilized Human CD3E&CD3D at 2ug/ml (100μl/Well). Dose response curve for Foralumab, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA.
The purity of Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.
Human CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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