Human VEGF165 Protein (VEG-HM065)

  • Express System: Expi293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 26.9ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Ala27-Arg191

Catalog: VEG-HM065

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Product Description Recombinant Human VEGF165 Protein is expressed from Expi293 without tag.It contains Ala27-Arg191.
Product Category Recombinant Protein
Target VEGF165
Synonyms VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609
Gene/Protein ID P15692-4
Species Human
Exact Sequence Ala27-Arg191
Molecular Weight The protein has a predicted MW of 19.2 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 26.9ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag No Tag
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 26.9ng/ml determined by ELISA (QC Test).
Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGFR2, mFc Tag with the EC50 of 42.3ng/ml determined by ELISA.
Human VEGF165 stimulates proliferation of human umbilical vein endothelial cells (HUVEC) with the ED50 for this effect is 2.57ng/mL (QC Test).
The purity of Human VEGF165 is greater than 95% as determined by SEC-HPLC.
Human VEGF165 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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