Name: Human VEGF165 Protein (VEG-HM065)
Brand: KactusBio
SKU: VEG-HM065-100UG
Price: 313.5 USD
Availability: InStock

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human VEGF165 at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 46.4ng/ml determined by ELISA.
Amino Acid Range: Ala27-Arg191

Catalog #VEG-HM065

Size

100UG

500UG

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Human VEGF165 Protein (VEG-HM065) Summary

Product Description	Recombinant Human VEGF165 Protein is expressed from HEK293 without tag.It contains Ala27-Arg191.
Product Background	Human papillomavirus (HPV) infection is an established risk factor for cervical carcinogenesis. VEGF165 was significantly higher, whereas VEGFC and VEGFD were significantly lower in malignant cervical carcinoma tissues as compared to normal cervix tissues. Expression levels of VEGF121 and VEGFC were significantly associated with type of tumor growth while VEGF165 was significantly associated with lymph node metastasis.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human VEGF165 Protein (VEG-HM065) Specifications/Details

Protein	VEGF165
Synonyms	VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165
Accession	P15692-4
Species	Human
Biotinylated	no
Amino Acid Range	Ala27-Arg191
Molecular Weight	The protein has a predicted MW of 19.2 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human VEGF165 at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 46.4ng/ml determined by ELISA.
Product Tag	No Tag
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.05 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human VEGF165 at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 46.4ng/ml determined by ELISA.
	Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, HisTag (Cat. VGF-HM1R2) with the EC50 of 0.35ug/ml determined by ELISA.
	Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Neuropilin-1, His Tag (Cat. NRP-HM101) with the EC50 of 1.26ug/ml determined by ELISA.
	Immobilized Human VEGF165, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
	Human VEGF165 on Bis-Tris PAGE under reduced condition and Non reduced (N) condition. The purity is greater than 95%.
	Measured by a reporter gene assay using HEK293T-KDR-NFAT Cell line. The ED50-for this effect is < 10 ng/mL (QC Test).
	Serial dilutions of Anti-VEGF165 Antibody were added into Human VEGF165, No Tag: Biotinylated Human VEGF R2, His Avi Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.30ug/ml.
	The purity of Human VEGF165 is greater than 95% as determined by SEC-HPLC.

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