Human VEGF165 Protein (VEG-HM065)

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human VEGF165 at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGFR2, mFc Tag with the EC50 of 42.3ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala27-Arg191

Catalog: VEG-HM065

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Product Description	Recombinant Human VEGF165 Protein is expressed from HEK293 without tag.It contains Ala27-Arg191.
Product Background	Human papillomavirus (HPV) infection is an established risk factor for cervical carcinogenesis. VEGF165 was significantly higher, whereas VEGFC and VEGFD were significantly lower in malignant cervical carcinoma tissues as compared to normal cervix tissues. Expression levels of VEGF121 and VEGFC were significantly associated with type of tumor growth while VEGF165 was significantly associated with lymph node metastasis.
Product Category	Recombinant Protein

Target	VEGF165
Synonyms	VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609
Gene/Protein ID	P15692-4
Species	Human
Exact Sequence	Ala27-Arg191
Molecular Weight	The protein has a predicted MW of 19.2 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human VEGF165 at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGFR2, mFc Tag with the EC50 of 42.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	No Tag
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human VEGF165 at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGFR2, mFc Tag with the EC50 of 42.3ng/ml determined by ELISA.
	Immobilized Human VEGF165 at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 28.7ng/ml determined by ELISA (QC Test).
	Human VEGF165 stimulates proliferation of human umbilical vein endothelial cells (HUVEC) with the ED50 for this effect is 2.57ng/mL (QC Test).
	The purity of Human VEGF165 is greater than 95% as determined by SEC-HPLC.
	Human VEGF165 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.