Human IFN gamma/IFNG Protein (IFN-HM00G)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IFN gamma, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 27.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln24-Gln166
Catalog #IFN-HM00G
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Product Description
 Recombinant Human IFN gamma/IFNG Protein is expressed from E.coli without tag.It contains Gln24-Gln166.
Product Background
 Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease.
Product Category
 Recombinant Protein
Target
 IFN gamma/IFNG
Accession
Species
 Human
Amino Acid Range
 Gln24-Gln166
Molecular Weight
 The protein has a predicted MW of 16.77 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Human IFN gamma, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 27.6ng/ml determined by ELISA. See testing image for detail.
Product Tag
 No Tag
Express System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 50mM Tris (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IFN gamma, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 27.6ng/ml determined by ELISA.
Human IFN gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IFN gamma is greater than 95% as determined by SEC-HPLC.
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