Human EGF Protein (EGF-HE001)

  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human EGF, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.82ug/ml determined by ELISA (QC Test). The affinity constant of 0.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Asn971-Arg1023

Catalog: EGF-HE001

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Product Description Recombinant Human EGF Protein is expressed from E.coli without tag.It contains Asn971-Arg1023.

Product Background

The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.

Product Category

Recombinant Protein

Target

EGF
Synonyms HOMG4; EGF; URG
Accession

Species

Human

Exact Sequence

Asn971-Arg1023
Molecular Weight The protein has a predicted MW of 5.6 kDa same as Tris-Bis PAGE result.

Activity

Immobilized Human EGF, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.82ug/ml determined by ELISA (QC Test). The affinity constant of 0.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

No Tag

Express System

E.coli
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 0.2EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human EGFR, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human EGF, No Tag with an affinity constant of 0.18 nM as determined in SPR assay (Biacore T200).
Immobilized Human EGF, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.82ug/ml determined by ELISA (QC Test).
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50-for this effect is typically 0.1 - 0.2 ng/mL (QC Test).
The purity of Human EGF is greater than 95% as determined by SEC-HPLC.
Human EGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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