Human EGF Protein (EGF-HE001)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human EGF, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.82ug/ml determined by ELISA (QC Test). The affinity constant of 0.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asn971-Arg1023
Catalog #EGF-HE001
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Product Description
 Recombinant Human EGF Protein is expressed from E.coli without tag.It contains Asn971-Arg1023.
Product Background
 The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
Product Category
 Recombinant Protein
Target
 EGF
Synonyms
 HOMG4; EGF; URG
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Asn971-Arg1023
Molecular Weight
 The protein has a predicted MW of 5.6 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Human EGF, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.82ug/ml determined by ELISA (QC Test). The affinity constant of 0.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 No Tag
Express System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.2EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human EGFR, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human EGF, No Tag with an affinity constant of 0.18 nM as determined in SPR assay (Biacore T200).
Immobilized Human EGF, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.82ug/ml determined by ELISA (QC Test).
Human EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50-for this effect is typically 0.1 - 0.2 ng/mL (QC Test).
The purity of Human EGF is greater than 95% as determined by SEC-HPLC.
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