Name: Human EGF Protein (EGF-HE001)
Brand: KactusBio
SKU: EGF-HE001-100UG
Price: 24.0 USD
Availability: InStock

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human EGF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 1.56ug/ml determined by ELISA (QC Test). The affinity constant of 1.33 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Asn971-Arg1023

Catalog #EGF-HE001

Size

100UG

500UG

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Human EGF Protein (EGF-HE001) Summary

Product Description	Recombinant Human EGF Protein is expressed from E.coli without tag.It contains Asn971-Arg1023.
Product Background	The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human EGF Protein (EGF-HE001) Specifications/Details

Protein	EGF
Synonyms	HOMG4; EGF; URG
Accession	P01133-1
Species	Human
Biotinylated	no
Amino Acid Range	Asn971-Arg1023
Molecular Weight	The protein has a predicted MW of 5.6 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human EGF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 1.56ug/ml determined by ELISA (QC Test). The affinity constant of 1.33 nM as determined in SPR assay (Biacore T200).
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.2 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, No Tag with an affinity constant of 1.33 nM as determined in SPR assay (Biacore T200).
	Immobilized Human EGF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 1.56ug/ml determined by ELISA (QC Test).
	Human EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50-for this effect is typically 0.1 - 0.2 ng/mL (QC Test).
	The purity of Human EGF is greater than 95% as determined by SEC-HPLC.