- Express System: HEK293
 - Product Tag: C-His-Avi
 - Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
 - Activity: The affinity constant of 1.67 uM as determined in SPR assay (Biacore T200).
 - Amino Acid Range: Asp25-Leu609
 
Product Description | 
                Recombinant Biotinylated Human Serum Albumin Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp25-Leu609. | 
Product Background | 
              Human serum albumin (HSA), the most prominent protein in plasma, binds different classes of ligands at multiple sites. HSA provides a depot for many compounds, affects pharmacokinetics of many drugs, holds some ligands in a strained orientation providing their metabolic modification, renders potential toxins harmless transporting them to disposal sites, accounts for most of the antioxidant capacity of human serum, and acts as a NO-carrier. | 
Product Category | 
    Recombinant Protein / Antibody discovery / Antibody discovery | Others | 
Protein  | 
              Serum Albumin | 
Synonyms  | 
              Albumin; ALB; Serum albumin; ANALBA; FDAH; PRO0883; PRO0903; PRO1341 | 
Accession  | 
              |
Species  | 
              Human | 
Biotinylated  | 
              yes | 
Amino Acid Range  | 
              Asp25-Leu609 | 
Molecular Weight  | 
              The protein has a predicted MW of 69.4 kDa. Due to glycosylation, the protein migrates to 69-70 kDa based on Bis-Tris PAGE result. | 
Activity  | 
              The affinity constant of 1.67 uM as determined in SPR assay (Biacore T200). | 
Product Tag  | 
              C-His-Avi | 
Expression System  | 
              HEK293 | 
Purity  | 
              > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC | 
Endotoxin  | 
              Less than 1 EU per ug by the LAL method. | 
Form  | 
            Lyophilized | 
Shipping  | 
            Shipped at ambient temperature. | 
Formulation  | 
            Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. | 
Reconstitution  | 
            Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. | 
Stability And Storage  | 
            -20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. | 
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