Biotinylated Human Serum Albumin Protein (BSA-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: The affinity constant of 1.67 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asp25-Leu609
Catalog #BSA-HM401B
Regular price $150.00
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Product Description
Recombinant Biotinylated Human Serum Albumin Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp25-Leu609.
Product Background
Human serum albumin (HSA), the most prominent protein in plasma, binds different classes of ligands at multiple sites. HSA provides a depot for many compounds, affects pharmacokinetics of many drugs, holds some ligands in a strained orientation providing their metabolic modification, renders potential toxins harmless transporting them to disposal sites, accounts for most of the antioxidant capacity of human serum, and acts as a NO-carrier.
Product Category
Recombinant Protein
Target
Serum Albumin
Synonyms
Albumin; ALB; Serum albumin; ANALBA; FDAH; PRO0883; PRO0903; PRO1341
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Asp25-Leu609
Molecular Weight
The protein has a predicted MW of 69.4 kDa. Due to glycosylation, the protein migrates to 69-70 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 1.67 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human Serum Albumin, His-Avi Tag immobilized on CM5 Chip can bind Human FcRn, His Tag with an affinity constant of 1.67 uM as determined in SPR assay (Biacore T200).
Biotinylated Human Serum Albumin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Serum Albumin is greater than 95% as determined by SEC-HPLC.

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