Human APRIL/TNFSF13 Trimer Protein (APR-HM110)

  • Express System: HEK293
  • Product Tag: N-His-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human APRIL Trimer, His Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Human BCMA, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Lys112-Leu250

Catalog: APR-HM110

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Product Description Recombinant Human APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Lys112-Leu250.

Product Background

The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.

Product Category

Recombinant Protein

Target

APRIL/TNFSF13 Trimer
Synonyms CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik
Accession

Species

Human

Amino Acid Range

Lys112-Leu250
Molecular Weight The protein has a predicted MW of 49.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human APRIL Trimer, His Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Human BCMA, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His-Flag

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human APRIL Trimer, His Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Human BCMA, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA.
The purity of Human APRIL Trimer is greater than 95% as determined by SEC-HPLC.
Human APRIL Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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