Human APRIL/TNFSF13 Trimer Protein (APR-HM110)

  • Express System: HEK293
  • Product Tag: N-His-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human APRIL Trimer, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Human BCMA, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Lys112-Leu250

Catalog: APR-HM110

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Product Description Recombinant Human APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Lys112-Leu250.
Product Category Recombinant Protein
Target APRIL/TNFSF13 Trimer
Synonyms CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik
Gene/Protein ID O75888-1
Species Human
Exact Sequence Lys112-Leu250
Molecular Weight The protein has a predicted MW of 49.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human APRIL Trimer, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Human BCMA, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Flag
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human APRIL Trimer, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Human BCMA, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
The purity of Human APRIL Trimer is greater than 95% as determined by SEC-HPLC.
Human APRIL Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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